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EAAC1 Antibody (MA5-45547) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with EAAC1 monoclonal antibody (Product # MA5-45547) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red... View More
Product Details
MA5-45547
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N180/41
Immunogen
Fusion protein amino acids 1-524 (full length) of human EAAC1
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932001
Product Specific Information
5 µg of MA5-45547 was sufficient for immunoprecipitation of EAAC1 from 50 µg of rat brain lysate.|Detects approximately 60kDa.
This antibody was formerly sold as clone S180-41.
Target Information
L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. To maintain glutamate levels below excitoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identified: the sodium-dependent GLAST-1, GLT-1, EAAC-1 and the chloride-dependent EAAT-4. EAAC-1 is expressed in the CNS and also found in kidney and small intestine. Structural features of glutamate transporters are believed to include eight membrane-spanning alpha-helices and a loop-pore structure which is unique among secondary transporters but may resemble loop-pores found in ion channels. A second distinctive structural feature is the presence of a highly amphipathic membrane-spanning helix that provides a hydrophilic path through the membrane. Mice with homozygous deletions of the EAAC-1 transporter gene develop dicarboxylic aminoaciduria and display reduced spontaneous locomotor activity although no neurodegeneration was observed over a period of 12 months.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: excitatory amino acid carrier 1; Excitatory amino acid transporter 3; excitatory amino acid transporter-3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1; Solute carrier family 1 A1 (brain glutamate transporter); Solute carrier family 1 member 1; solute carrier family 1, member 1
Gene Aliases: DCBXA; EAAC1; EAAT3; HEAAC1; REAAC1; SCZD18; SLC1A1
UniProt ID: (Human) P43005, (Rat) P51907
Entrez Gene ID: (Human) 6505, (Rat) 25550
L-glutamate transmembrane transporter activity
high-affinity glutamate transmembrane transporter activity
protein binding
amino acid transmembrane transporter activity
glutamate:sodium symporter activity
glutamate binding
cysteine transmembrane transporter activity
sodium:dicarboxylate symporter activity
primary active transporter
ion transport
chemical synaptic transmission
positive regulation of heart rate
glutamate secretion
cysteine transport
protein homooligomerization
L-glutamate import
D-aspartate import
L-glutamate transmembrane transport
cysteine transmembrane transport
L-glutamate transport
sodium ion transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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