|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to human EAAT2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Hydroxyl Side Chain of a Highly Conserved Serine Residue Is Required for Cation Selectivity and Substrate Transport in the Glial Glutamate Transporter GLT-1/SLC1A2.
PA5-17099 was used in western blot to study the importance of serine 364 on glutamate transporter function
|Simonin A,Montalbetti N,Gyimesi G,Pujol-Giménez J,Hediger MA||The Journal of biological chemistry (290:30464)||2015|
HFE polymorphisms affect cellular glutamate regulation.
PA5-17099 was used in western blot to examine the role of HFE polymorphisms in neurodegenerative diseases
|Mitchell RM,Lee SY,Simmons Z,Connor JR||Neurobiology of aging (32:1114)||2011|
Functional expression, purification and high sequence coverage mass spectrometric characterization of human excitatory amino acid transporter EAAT2.
PA5-17099 was used in western blot to investigate a new efficient approach to produce and characterize human excitatory amino acid transporter 2
|Ye R,Rhoderick JF,Thompson CM,Bridges RJ||Protein expression and purification (74:49)||2010|