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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Bacterially produced, recombinant, full-length human NHERF1.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
For use in Western blot applications, MA1-19291 should be placed in a vertical incubator for 60 minutes. A suggested positive control for this product is RAJI human lymphoma cell lysate.
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunohistochemical analysis of ezrin-radixin-moesin-binding phosphoprotein 50 in prostatic adenocarcinoma.
MA1-19291 was used in immunohistochemistry to investigate the expression of ezrin-radixin-moesin-binding phosphoprotein 50 in normal and pathological tissues
|Bartholow TL,Becich MJ,Chandran UR,Parwani AV||BMC urology (11:null)||2011|
EBP50; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-binding phosphoprotein 50; Na+/H+ exchange regulatory co-factor; regulatory cofactor of Na(+)/H(+) exchanger; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
EBP50; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1