Invitrogen

EBP50 Recombinant Rabbit Monoclonal Antibody (JJ0946)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Protocols

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EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of EBP50 of paraffin-embedded Human kidney tissue using a EBP50 Monoclonal antibody (Product #MA5-32493). Counter stained with hematoxylin.

EBP50 Antibody in Western Blot (WB) — Western blot was performed using Anti-EBP50 Monoclonal Antibody (Product # MA5-32493) and a 50 kDa band corresponding to EBP50 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of T-47D (Lane 1), SK-BR-3 (Lane 2), MDA-MB-231 (Lane 3), HeLa (Lane 4), Hep G2 (Lane 5) and PC-12 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

EBP50 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of EBP50 was observed in SKBR3 and T-47D in higher levels in comparison to MDA-MB231 in low levels using Anti-EBP50 Monoclonal Antibody (Product # PA5-32493) in Western Blot. (doi:https://doi.org/10.3892/or.2017.5649). {RE}

Product Details

MA5-32493

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunohistochemistry (IHC)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Expression System	HEK293 cells
Class	Recombinant Monoclonal
Type	Antibody
Clone	JJ0946
Immunogen	Recombinant protein within Human EBP50 aa 1-150 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809770

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EBP50; ERM-binding phosphoprotein; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

Gene Aliases: EBP50; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1

UniProt ID: (Human) O14745, (Rat) Q9JJ19

Entrez Gene ID: (Human) 9368, (Rat) 59114

Function(s)

Process(es)

receptor binding protein binding beta-catenin binding chloride channel regulator activity phosphatase binding PDZ domain binding beta-2 adrenergic receptor binding protein complex binding protein complex scaffold protein self-association dopamine receptor binding growth factor receptor binding protein domain specific binding

EBP50 Recombinant Rabbit Monoclonal Antibody (JJ0946)

Product Details

MA5-32493

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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