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Invitrogen

EBP50 Recombinant Rabbit Monoclonal Antibody (JJ0946)

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EBP50 Recombinant Rabbit Monoclonal Antibody (JJ0946)

Product Details

MA5-32493

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JJ0946

Immunogen

Recombinant protein within Human EBP50 aa 1-150.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809770

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cytoskeletal-linking protein; EBP50; ERM-binding phosphoprotein; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor 1; Na+/H+ exchange regulatory co-factor; NHE-RF; NHERF-1; regulatory cofactor of Na(+)/H(+) exchanger; SLC9A3 regulator 1; So; sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1; unnamed protein product

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Gene Aliases: EBP50; NHE-RF; NHERF; NHERF-1; NPHLOP2; SLC9A3R1

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UniProt ID: (Human) O14745, (Rat) Q9JJ19

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Entrez Gene ID: (Human) 9368, (Rat) 59114

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Function(s)
receptor binding protein binding beta-catenin binding gamma-aminobutyric acid transmembrane transporter activity chloride channel regulator activity phosphatase binding PDZ domain binding beta-2 adrenergic receptor binding type 2 metabotropic glutamate receptor binding type 3 metabotropic glutamate receptor binding identical protein binding protein anchor dopamine receptor binding binding, bridging growth factor receptor binding channel activator activity protein domain specific binding ion channel binding macromolecular complex binding myosin II binding
Process(es)
morphogenesis of an epithelium plasma membrane organization nuclear migration negative regulation of cell proliferation regulation of cell shape regulation of cell size negative regulation of platelet-derived growth factor receptor signaling pathway gland morphogenesis microvillus assembly bile acid secretion glutathione transport cilium organization establishment of epithelial cell apical/basal polarity maintenance of epithelial cell apical/basal polarity regulation of protein kinase activity negative regulation of mitotic cell cycle establishment of Golgi localization negative regulation of protein kinase B signaling gamma-aminobutyric acid import protein-containing complex assembly renal absorption negative regulation of ERK1 and ERK2 cascade protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway cerebrospinal fluid circulation renal phosphate ion absorption import across plasma membrane transport across blood-brain barrier negative regulation of cell motility positive regulation of intrinsic apoptotic signaling pathway renal sodium ion transport adenylate cyclase-activating dopamine receptor signaling pathway sensory perception of sound protein localization negative regulation of sodium ion transport actin cytoskeleton organization negative regulation of cell migration cellular phosphate ion homeostasis positive regulation of ion transmembrane transport auditory receptor cell stereocilium organization phospholipase C-activating dopamine receptor signaling pathway regulation of renal phosphate excretion
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