Invitrogen

ECH1 Monoclonal Antibody (9D11AF3)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (12) ECH1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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ECH1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of ECH1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ECH1 (9D11AF3) Mouse Monoclonal Antibody (Product # A21972) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ECH1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with ECH1 siRNA and loss of signal was observed in immunofluorescence application using ECH1 Mouse Monoclonal antibody (Product # A21972). {KD}

Product Details

A21972

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	5 µg/mL	-
ELISA (ELISA)	Assay-dependent	-
Immunoprecipitation (IP)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	9D11AF3
Immunogen	ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to the peroxisome
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	purified
Storage buffer	HEPES buffered saline
Contains	0.02% sodium azide
Storage conditions	4° C
RRID	AB_2535884

Target Information

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta3,5-delta2,4-dienoyl-CoA isomerase; delta3,5-delta2,4-dienoyl-coenzyme A isomerase; dienoyl-CoA isomerase; enoyl CoA hydratase 1, peroxisomal; enoyl Coenzyme A hydratase 1, peroxisomal; enoyl hydratase-like protein peroxisomal; enoyl hydratase-like protein, peroxisomal; enoyl-CoA hydratase 1, peroxisomal; Peroxisomal enoyl hydratase-like protein; peroxisomal enoyl-CoA hydratase 1

Gene Aliases: ECH1; HPXEL; Pxel

UniProt ID: (Human) Q96EZ9, (Rat) Q62651

Entrez Gene ID: (Human) 1891, (Rat) 64526

Molecular Function: hydratase lyase metabolite interconversion enzyme