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|Tested species reactivity||Human , Mouse|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 ug/ml|
|Immunohistochemistry (IHC)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1040 detects S1P1 protein in transfected human and mouse cell samples. This antibody shows no cross-reactivity to S1P3.
PA1-1040 has successfully been used in immuno-fluorescence, immunohistochemistry, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. Immunofluorescence data demonstrates that S1P1 is localized to the plasma membrane after staining with PA1-1040.
The PA1-1040 immunogen is a synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. This sequence is completely conserved in rat and 92% conserved in human S1P1. This peptide (Cat. # PEP-220) is available for use in neutralization and control experiments.
Sphingosine 1-phosphate (S1P) is bioactive lipid derived from the metabolism of sphingomyelin. S1P is predominately synthesized, stored, and released from platelets. When released, this molecule stimulates signaling pathways via G-protein coupled receptors. One such receptor is the heterotrimeric guanine nucleotide binding protein shingosine 1-phosphate receptor type 1 (S1P1,also known as EDG1).
Research has demonstrated that S1P regulates numerous cellular responses including, wound healing, cell growth, prevention of apoptosis, vascular maturation and cellular migration and survival. In studies involving S1P1 deficient embryos, blood vessels where incompletely maturated with smooth muscles cells, resulting in embryonic death. In contrast, a separate study demonstrated that overexpression of S1P1 led to the stimulation of cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Stress-induced production of chemokines by hair follicles regulates the trafficking of dendritic cells in skin.
PA1-1040 was used in immunohistochemistry to study the regulatory role of stress-induced chemokines on dendritic cell trafficking in skin
|Nagao K,Kobayashi T,Moro K,Ohyama M,Adachi T,Kitashima DY,Ueha S,Horiuchi K,Tanizaki H,Kabashima K,Kubo A,Cho YH,Clausen BE,Matsushima K,Suematsu M,Furtado GC,Lira SA,Farber JM,Udey MC,Amagai M||Nature immunology (13:744)||2012|
A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart.
PA1-1040 was used in western blot to investigate the effect of the polysaccharide MDG-1 on specific gene expression and cell survival in ischemic hearts
|Wang S,Zhang Z,Lin X,Xu DS,Feng Y,Ding K||Glycobiology (20:473)||2010|
S1p1, Lpb1, AI849002, CHEDG1, S1p, ECGF1, CD363, D1S3362, Edg1, S1P1, EDG1, EDG-1
S1P receptor 1, S1P receptor Edg-1, endothelial differentiation G-protein coupled receptor 1, endothelial differentiation sphingolipid G-protein-coupled receptor 1, lysophospholipid receptor B1, sphingosine 1-phosphate receptor 1, sphingosine 1-phosphate receptor Edg-1, endothelial differentiation, sphingolipid G-protein-coupled receptor, 1, sphingosine 1-phosphate receptor EDG1, Sphingolipid Receptor 1, Endothelial Differentiation Gene 1, EDG1