|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic 19 amino acid peptide from N-Terminus of human EDG3 / S1P3.|
|Purification||Antigen affinity chromatography|
|Contains||< 0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In IHC applications, antigen retrieval using Proteinase K treatment is recommended.
Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey, Marmoset (95%).
EDG-3 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-3 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-3 is expressed in cardiovascular, leukocyte-containing and other tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.