Western blot analysis of EED using A) 30 µg Neuro2A whole lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate and E) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an EED monoclonal antibody (Product # MA5-16314) at a dilution of 1:1000.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant fragment containing a sequence corresponding to a region within amino acids 1 and 269 of EED|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-16314 targets EED in WB applications and shows reactivity with Human samples.
The MA5-16314 immunogen is recombinant fragment containing a sequence corresponding to a region within amino acids 1 and 269 of EED
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene.
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