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Invitrogen
EEF1G Recombinant Rabbit Monoclonal Antibody (JE54-03)
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FIGURE: 1 / 11
EEF1G Antibody (MA5-36168) in ICC/IF
Immunocytochemical analysis of EEF1G in SKOV-3 cells using a EEF1G monoclonal antibody (Product #MA5-36168). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.
Product Details
MA5-36168
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE54-03
Immunogen
Recombinant protein within Human Elongation factor 1-gamma aa 1-163
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2890440
Target Information
EF-1 (elongation factor-1) is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. EF-1 gamma (elongation factor- 1 gamma), also known as EEF1G or GIG35, is a 437 amino acid subunit of the EF-1 complex. Expressed in stomach, pancreas, brain, lung, kidney, intestine, liver and spleen, EF-1 gamma contains an N-terminal Glutathione transferase domain which is thought to be involved in anchoring the complex to various cellular components. Additionally, EF-1 gamma may play a key role in the assembly of multiprotein complexes containing aminoacyl-tRNA synthetases. Increased expression of EF-1 gamma is associated with pancreatic cancer, suggesting a possible role for EF-1 gamma in the oncogenic transformation process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eEF-1B gamma; EF-1-gamma; Elongation factor 1-gamma; pancreatic tumor-related protein; PRO1608; translation elongation factor eEF-1 gamma chain
Gene Aliases: 2610301D06Rik; AA407312; EEF1G; EF1G; GIG35; PRO1608
UniProt ID: (Human) P26641, (Rat) Q68FR6, (Mouse) Q9D8N0
Entrez Gene ID: (Human) 1937, (Rat) 293725, (Mouse) 67160
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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