Invitrogen

EEF1G Recombinant Rabbit Monoclonal Antibody (JE54-03)

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EEF1G Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of EEF1G in SKOV-3 cells using a EEF1G monoclonal antibody (Product #MA5-36168). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.

EEF1G Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of EEF1G in paraffin-embedded human colon carcinoma tissue using a monoclonal antibody (Product #MA5-36168). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

EEF1G Antibody in Western Blot (WB) — Western blot analysis of EEF1G using a EEF1G monoclonal antibody (Product #MA5-36168). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse thymus tissue lysate, Lane 2: rat spleen tissue lysate, Lane 3: mouse ovary tissue lysate, Lane 4: rat thymus tissue lysate

EEF1G Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of EEF1G using Daudi cells and a EEF1G monoclonal antibody (Product #MA5-36168). The cells were fixed, permeabilized and stained with the primary antibody at a dilution of 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Product Details

MA5-36168

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	-
Flow Cytometry (Flow)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JE54-03
Immunogen	Recombinant protein within Human Elongation factor 1-gamma aa 1-163 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2890440

Target Information

EF-1 (elongation factor-1) is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. EF-1 gamma (elongation factor- 1 gamma), also known as EEF1G or GIG35, is a 437 amino acid subunit of the EF-1 complex. Expressed in stomach, pancreas, brain, lung, kidney, intestine, liver and spleen, EF-1 gamma contains an N-terminal Glutathione transferase domain which is thought to be involved in anchoring the complex to various cellular components. Additionally, EF-1 gamma may play a key role in the assembly of multiprotein complexes containing aminoacyl-tRNA synthetases. Increased expression of EF-1 gamma is associated with pancreatic cancer, suggesting a possible role for EF-1 gamma in the oncogenic transformation process.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eEF-1B gamma; EF-1-gamma; Elongation factor 1-gamma; pancreatic tumor-related protein; PRO1608; translation elongation factor eEF-1 gamma chain

Gene Aliases: 2610301D06Rik; AA407312; EEF1G; EF1G; GIG35; PRO1608

UniProt ID: (Human) P26641, (Rat) Q68FR6, (Mouse) Q9D8N0

Entrez Gene ID: (Human) 1937, (Rat) 293725, (Mouse) 67160

Function(s)

translational elongation response to virus glutathione metabolic process cell-cell adhesion translation

Process(es)

translation elongation factor activity glutathione transferase activity protein binding cadherin binding involved in cell-cell adhesion