Immunocytochemistry analysis of Human PBMCs stained with ABfinity™ EGF Recombinant Rabbit Monoclonal Antibody using Alexa Fluor® 488 goat anti-rabbit as a secondary antibody ("a", green) and DAPI to stain the nuclei ("b", blue). A composite image of cells showing nuclear and epidermal localization of EGF is also shown ("c").
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.