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Immunofluorescence analysis of EGFR was done on 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled EGFR (111.6) Mouse Monoclonal Antibody (MA513269) at 2ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Dog, Primate, Sheep, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Extracellular domain of human recombinant EGFR protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 5 publications below|
|Immunocytochemistry (ICC)||See 7 publications below|
|Immunohistochemistry (IHC)||See 30 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Flow Cytometry (Flow)||See 3 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Gel Shift (GS)||See 1 publications below|
MA5-13269 targets Epidermal Growth Factor Receptor in IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-13269 immunogen is extracellular domain of human recombinant EGFR protein.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
IP-MS enrichment of EGFR (LFQ intensity): EGFR was enriched 74-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and EGFR antibody (Part No. MA5-13269). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An early endosome regulator, Rab5b, is an LRRK2 kinase substrate.
MA5-13269 was used in western blot to elucidate the relationship between LRRK2 and Rab5b
|Yun HJ,Kim H,Ga I,Oh H,Ho DH,Kim J,Seo H,Son I,Seol W||Journal of biochemistry (157:485)||2015|
Structure-function analysis of nucleolin and ErbB receptors interactions.
MA5-13269 was used in western blot to investigate the interaction between nucleolin and ErbB receptors
|Farin K,Di Segni A,Mor A,Pinkas-Kramarski R||PloS one (4:null)||2009|
EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2.
MA5-13269 was used in western blot to examine the role of EGFR and ADAMs in the endocytic trafficking of the desmosomal cadherin desmoglein 2
|Klessner JL,Desai BV,Amargo EV,Getsios S,Green KJ||Molecular biology of the cell (20:328)||2009|
Geldanamycin-associated inhibition of intracellular trafficking is attributed to a co-purified activity.
MA5-13269 was used in western blot to study a co-purifying contaminant of geldanamycin preparations as the component inhibiting intracellular trafficking
|Barzilay E,Ben-Califa N,Supino-Rosin L,Kashman Y,Hirschberg K,Elazar Z,Neumann D||The Journal of biological chemistry (279:6847)||2004|
A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera.
MA5-13269 was used in western blot to develop an acridium-linked immunosorbent assay for measuring soluble ErbB1 in human serum
|Baron AT,Lafky JM,Connolly DC,Peoples J,O'Kane DJ,Suman VJ,Boardman CH,Podratz KC,Maihle NJ||Journal of immunological methods (219:23)||1998|
EGFR, HER2 and HER3 dimerization patterns guide targeted inhibition in two histotypes of esophageal cancer.
MA5-13269 was used in immunocytochemistry and immunohistochemistry to study the value of the expression and dimerization patterns of EGFR, HER2 and HER3 in esophageal cancer histotypes for guiding targeted anti-EGFR and -HER2 therapy
|Fichter CD,Timme S,Braun JA,Gudernatsch V,Schöpflin A,Bogatyreva L,Geddert H,Faller G,Klimstra D,Tang L,Hauschke D,Werner M,Lassmann S||International journal of cancer (135:1517)||2014|
Prostaglandin E1 and misoprostol increase epidermal growth factor production in 3D-cultured human annulus cells.
MA5-13269 was used in immunocytochemistry to investigate the influence of PGE1 and misoprostol on epidermal growth factor expression in 3D-cultured human annulus cells
|Gruber HE,Hoelscher G,Loeffler B,Chow Y,Ingram JA,Halligan W,Hanley EN||The spine journal : official journal of the North American Spine Society (9:760)||2009|
Ultraviolet irradiation-induces epidermal growth factor receptor (EGFR) nuclear translocation in human keratinocytes.
MA5-13269 was used in immunocytochemistry to investigate the induction of EGFR nuclear translocation by ultraviolet irradiation in human Keratinocytes
|Xu Y,Shao Y,Zhou J,Voorhees JJ,Fisher GJ||Journal of cellular biochemistry (107:873)||2009|
Dual-mode reflectance and fluorescence near-video-rate confocal microscope for architectural, morphological and molecular imaging of tissue.
MA5-13269 was used in immunocytochemistry to develop a dual-mode reflectance and fluorescence confocal microscope for imaging tissue samples with high resolution
|Carlson AL,Coghlan LG,Gillenwater AM,Richards-Kortum RR||Journal of microscopy (228:11)||2007|
Fluorescent nanocrystals for use in early cervical cancer detection.
MA5-13269 was used in immunocytochemistry to evaluate quantum dots for early cervical cancer detection
|Nida DL,Rahman MS,Carlson KD,Richards-Kortum R,Follen M||Gynecologic oncology (99:S89)||2005|
Characterization of a transplantable hormone-responsive human prostatic cancer xenograft TEN12 and its androgen-resistant sublines.
MA5-13269 was used in immunocytochemistry to investigate the effectiveness of Xenograft TEN12 and its sublines on the progression of prostatic cancer
|Harper ME,Goddard L,Smith C,Nicholson RI||The Prostate (58:13)||2004|
Retroperitoneal cystic papillary carcinoma of probable nephrogenic origin.
MA5-13269 was used in immunocytochemistry to report on a case of retroperitoneal cystic papillary carcinoma of likely nephrogenic origin
|Praetorius L,Krogh J,Horn T||Scandinavian journal of urology and nephrology (35:147)||2001|
Role of EGFR as a prognostic factor for survival in head and neck cancer: a meta-analysis.
MA5-13269 was used in immunohistochemistry to study the prognostic value of EGFR in head and neck cancers
|Keren S,Shoude Z,Lu Z,Beibei Y||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (35:2285)||2014|
EGFR, HER-2 and KRAS in canine gastric epithelial tumors: a potential human model?
MA5-13269 was used in immunohistochemistry to examine canine gastric epithelial tumors for the expression of EGFR, HER-2 and KRAS in order to determine whether dog is a suitable model of the corresponding human cancer
|Terragni R,Casadei Gardini A,Sabattini S,Bettini G,Amadori D,Talamonti C,Vignoli M,Capelli L,Saunders JH,Ricci M,Ricci M,Ulivi P,Ulivi P||PloS one (9:null)||2014|
Single cell stochastic regulation of pilus phase variation by an attenuation-like mechanism.
MA5-13269 was used in immunohistochemistry to study the molecular mechanisms underlying pilus phase variation
|Danne C,Dubrac S,Trieu-Cuot P,Dramsi S||PLoS pathogens (10:null)||2014|
No-cost manual method for preparation of tissue microarrays having high quality comparable to semiautomated methods.
MA5-13269 was used in immunohistochemistry to optimize a manual method for the production of cheap, high quality tissue microarrays
|Foda AA||Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry (21:271)||2013|
Epidermal growth factor receptor as a biomarker for cervical cancer.
MA5-13269 was used in immunohistochemistry to investigate the expression of epidermal growth factor receptor in cervical cancer
|Soonthornthum T,Arias-Pulido H,Joste N,Lomo L,Muller C,Rutledge T,Verschraegen C||Annals of oncology : official journal of the European Society for Medical Oncology / ESMO (22:2166)||2011|
An aCGH classifier derived from BRCA1-mutated breast cancer and benefit of high-dose platinum-based chemotherapy in HER2-negative breast cancer patients.
MA5-13269 was used in immunohistochemistry to investigate an aCGH classifier for BRCA1 loss in breast cancer
|Vollebergh MA,Lips EH,Nederlof PM,Wessels LF,Schmidt MK,van Beers EH,Cornelissen S,Holtkamp M,Froklage FE,de Vries EG,Schrama JG,Wesseling J,van de Vijver MJ,van Tinteren H,de Bruin M,Hauptmann M,Rodenhuis S,Linn SC||Annals of oncology : official journal of the European Society for Medical Oncology / ESMO (22:1561)||2011|
Identification of different subtypes of breast cancer using tissue microarray.
MA5-13269 was used in immunohistochemistry to evaluate tissue microarray for classification of human breast cancer
|Munirah MA,Siti-Aishah MA,Reena MZ,Sharifah NA,Rohaizak M,Norlia A,Rafie MK,Asmiati A,Hisham A,Fuad I,Shahrun NS,Das S||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (52:669)||2011|
Transdifferentiation of glioblastoma cells into vascular endothelial cells.
MA5-13269 was used in immunohistochemistry to study the transdifferentiation of glioblastoma cells into vascular endothelial cells and the mechanism of resistance to anti-VEGF therapy.
|Soda Y,Marumoto T,Friedmann-Morvinski D,Soda M,Liu F,Michiue H,Pastorino S,Yang M,Hoffman RM,Kesari S,Verma IM||Proceedings of the National Academy of Sciences of the United States of America (108:4274)||2011|
Expression of epidermal growth factor receptor and vascular endothelial growth factor in vaginal squamous cell cancer.
MA5-13269 was used in immunohistochemistry to investigate VEGF and EGFR in vaginal squamous cell cancer
|Brunner A,Grimm C,Polterauer S,Hefler L,Stani J,Dudek G,Horvat R||American journal of obstetrics and gynecology (204:171.e1)||2011|
Comparison of four antibodies for immunohistochemical evaluation of epidermal growth factor receptor expression in non-small cell lung cancer.
MA5-13269 was used in immunohistochemistry to investigate the performance of four different anti-EGFR antibodies in immunohistochemical study of non-small cell lung cancer
|Mathieu A,Weynand B,Verbeken E,Da Silva S,Decaestecker C,Salmon I,Demetter P||Lung cancer (Amsterdam, Netherlands) (69:46)||2010|
Triple-negative breast cancer: immunohistochemical correlation with basaloid markers and prognostic value of survivin.
MA5-13269 was used in immunohistochemistry to study the prognostic value of survivin in patients with basaloid triplet-negative breast cancer
|Dogu GG,Ozkan M,Ozturk F,Dikilitas M,Er O,Ozturk A||Medical oncology (Northwood, London, England) (27:34)||2010|
Sex steroid and epidermal growth factor profile of giant meningiomas associated with pregnancy.
MA5-13269 was used in immunohistochemistry to investigate the distribution of specific proteins in giant meningiomas associated with pregnancy
|Hatiboglu MA,Cosar M,Iplikcioglu AC,Ozcan D||Surgical neurology (69:356)||2008|
TGFalpha and EGFR in ovine preimplantation embryos and effects on development.
MA5-13269 was used in immunohistochemistry to investigate the expression of transforming growth factor alpha and its receptor in ovine preimplantation embryos
|Zhou P,Liu DJ,Cang M,Ma YZ,Yang DS,Li HJ,Wang LM,Bou S,Feng HL||Animal reproduction science (104:370)||2008|
Gene expression profiling and histopathological characterization of triple-negative/basal-like breast carcinomas.
MA5-13269 was used in immunohistochemistry to study triple negative breast carcinoma histopathology and gene expression patterns in order to define clinically significant sub-groups
|Kreike B,van Kouwenhove M,Horlings H,Weigelt B,Peterse H,Bartelink H,van de Vijver MJ||Breast cancer research : BCR (9:null)||2008|
The role of heparin-binding EGF-like growth factor and amphiregulin in the epidermal proliferation of psoriasis in cooperation with TNFalpha.
MA5-13269 was used in immunohistochemistry to examine the role of growth factors in epidermal hyperplasia of psoriasis
|Yoshida A,Kanno H,Watabe D,Akasaka T,Sawai T||Archives of dermatological research (300:37)||2008|
Epidermal growth factor ligand/receptor loop and downstream signaling activation pattern in completely resected nonsmall cell lung cancer.
MA5-13269 was used in immunohistochemistry to study EGF receptor pathway activation in completely resected non-small cell lung cancer
|Volante M,Saviozzi S,Rapa I,Ceppi P,Cappia S,Calogero R,Novello S,Borasio P,Papotti M,Scagliotti GV||Cancer (110:1321)||2007|
Basaloid adenocarcinoma. A new variant of pulmonary adenocarcinoma.
MA5-13269 was used in immunohistochemistry to report a clinical case of basaloid adenocarcinoma
|Marci V,Volante M,Cappia S,Righi L,Novello C,Scagliotti GV,Brambilla E,Papotti M||Virchows Archiv : an international journal of pathology (451:729)||2007|
Co-expression of EGF receptor, TGFalpha and S6 kinase is significantly associated with colorectal carcinomas with distant metastases at diagnosis.
MA5-13269 was used in immunohistochemistry to study the expression EGF receptor, TGF alpha and S6 kinase as predictors of metastasis in colorectal cancer
|Tampellini M,Longo M,Cappia S,Bacillo E,Alabiso I,Volante M,Dogliotti L,Papotti M||Virchows Archiv : an international journal of pathology (450:321)||2007|
Downregulation of EGF receptor signaling in pancreatic islets causes diabetes due to impaired postnatal beta-cell growth.
MA5-13269 was used in immunohistochemistry and western blot to study the role of EGF receptor signaling in postnatal beta-cell growth in pancreatic islets
|Miettinen PJ,Ustinov J,Ormio P,Gao R,Palgi J,Hakonen E,Juntti-Berggren L,Berggren PO,Otonkoski T||Diabetes (55:3299)||2006|
Does the EGFR and VEGF expression predict the prognosis in colon cancer?
MA5-13269 was used in immunohistochemistry to examine the prognostic value of EGFR and VEGF expression for colon cancer
|Doger FK,Meteoglu I,Tuncyurek P,Okyay P,Cevikel H||European surgical research. Europa¿ische chirurgische Forschung. Recherches chirurgicales europe¿ennes (38:540)||2006|
Molecular subtypes of breast cancer and amplification of topoisomerase II alpha: predictive role in dose intensive adjuvant chemotherapy.
MA5-13269 was used in immunohistochemistry to study molecular subtypes of breast cancer and amplification of topoisomerase II alpha
|Hannemann J,Kristel P,van Tinteren H,Bontenbal M,van Hoesel QG,Smit WM,Nooij MA,Voest EE,van der Wall E,Hupperets P,de Vries EG,Rodenhuis S,van de Vijver MJ||British journal of cancer (95:1334)||2006|
Real-time detection of epidermal growth factor receptor expression in fresh oral cavity biopsies using a molecular-specific contrast agent.
MA5-13269 was used in immunohistochemistry to develop a novel targeted fluorescent contrast agent for EGFR expression in oral neoplasia
|Hsu ER,Gillenwater AM,Hasan MQ,Williams MD,El-Naggar AK,Richards-Kortum RR||International journal of cancer (118:3062)||2006|
Cyclooxygenase-2 (COX-2) levels before and after chemotherapy: a study in rectal cancer.
MA5-13269 was used in immunohistochemistry to study the immunohistochemical expression of Cox-2 before and after chemoradiation therapy of rectal cancer
|Watwe V,Javle M,Lawrence D,Groth J,Iyer R,El-Hajjar D,Geradts J||American journal of clinical oncology (28:560)||2005|
Gene expression and immunolocalization of heparin-binding epidermal growth factor-like growth factor and human epidermal growth factor receptors in human corpus luteum.
MA5-13269 was used in immunohistochemistry to study the expression and immunolocalization of HB-EGF-like growth factor and EGFR in human corpus luteum
|Akayama Y,Takekida S,Ohara N,Tateiwa H,Chen W,Nakabayashi K,Maruo T||Human reproduction (Oxford, England) (20:2708)||2005|
Epidermal growth factor receptor, protein kinase B/Akt, and glioma response to erlotinib.
MA5-13269 was used in immunohistochemistry to investigate the effect of erlotinib on the expression of epidermal growth factor receptor and other signaling components
|Haas-Kogan DA,Prados MD,Tihan T,Eberhard DA,Jelluma N,Arvold ND,Baumber R,Lamborn KR,Kapadia A,Malec M,Berger MS,Stokoe D||Journal of the National Cancer Institute (97:880)||2005|
Racial disparity of epidermal growth factor receptor expression in prostate cancer.
MA5-13269 was used in immunohistochemistry to study racial differences in EGFR expression in prostate cancer
|Shuch B,Mikhail M,Satagopan J,Lee P,Yee H,Chang C,Cordon-Cardo C,Taneja SS,Osman I||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (22:4725)||2004|
Inability of immunohistochemistry to predict clinical outcomes of endometrial cancer patients.
MA5-13269 was used in immunohistochemistry to study the inability of immunohistochemistry to predict clinical outcome in endometrial cancer
|Gossett DR,Alo P,Bristow RE,Galati M,Kyshtoobayeva A,Fruehauf J,Montz FJ||International journal of gynecological cancer : official journal of the International Gynecological Cancer Society (14:145)||2004|
In vitro chemoresistance and biomarker profiles are unique for histologic subtypes of epithelial ovarian cancer.
MA5-13269 was used in immunohistochemistry to study whether there is a relationship between histologic subtype of epithelial ovarian cancer and chemoresistance
|Cloven NG,Kyshtoobayeva A,Burger RA,Yu IR,Fruehauf JP||Gynecologic oncology (92:160)||2004|
Validation of tissue array technology in head and neck squamous cell carcinoma.
MA5-13269 was used in immunohistochemistry to evaluate the use of tissue array technology to analyse archived head and neck cancer tissue samples
|Chen B,van den Brekel MW,Buschers W,Balm AJ,van Velthuysen ML||Head & neck (25:922)||2003|
A pharmacodynamic study of the epidermal growth factor receptor tyrosine kinase inhibitor ZD1839 in metastatic colorectal cancer patients.
MA5-13269 was used in immunohistochemistry to study the pharmacodynamics of an EGFR tyrosine kinase inhibitor in metastatic colorectal cancer patients
|Daneshmand M,Parolin DA,Hirte HW,Major P,Goss G,Stewart D,Batist G,Miller WH,Matthews S,Seymour L,Lorimer IA||Clinical cancer research : an official journal of the American Association for Cancer Research (9:2457)||2003|
Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays.
MA5-13269 was used in blocking or activating experiment to develop a bead-based sandwich immunoassay for the profiling of receptor tyrosine kinase inhibitors
|Pötz O,Schneiderhan-Marra N,Henzler T,Herget T,Joos TO||Methods in molecular biology (Clifton, N.J.) (795:191)||2011|
MAP Kinase activation by receptor tyrosine kinases: in control of cell migration.
MA5-13269 was used in flow cytometry to review high throughput methods for profiling the activation of signalling molecules.
|Tarcic G,Yarden Y||Methods in molecular biology (Clifton, N.J.) (661:125)||2010|
Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments.
MA5-13269 was used in flow cytometry to study the use of yeast surface display for identifying stable folded protein domains and characterizing antibody binding epitopes
|Cochran JR,Kim YS,Olsen MJ,Bhandari R,Wittrup KD||Journal of immunological methods (287:147)||2004|
Profiling receptor tyrosine kinase activation by using Ab microarrays.
MA5-13269 was used in flow cytometry to study the receptor tyrosine kinase activation by through antibody microarrays
|Nielsen UB,Cardone MH,Sinskey AJ,MacBeath G,Sorger PK||Proceedings of the National Academy of Sciences of the United States of America (100:9330)||2003|
Engineering of PDMS surfaces for use in microsystems for capture and isolation of complex and biomedically important proteins: epidermal growth factor receptor as a model system.
MA5-13269 was used in immunoprecipitation to develop a novel system for capture and isolation of biomedically important proteins
|Lowe AM,Ozer BH,Wiepz GJ,Bertics PJ,Abbott NL||Lab on a chip (8:1357)||2008|
Using liquid crystals to report membrane proteins captured by affinity microcontact printing from cell lysates and membrane extracts.
MA5-13269 was used in immunoprecipitation to develop a liquid crystal surface for analysising membrane proteins in cell lysates and membrane extracts
|Jang CH,Tingey ML,Korpi NL,Wiepz GJ,Schiller JH,Bertics PJ,Abbott NL||Journal of the American Chemical Society (127:8912)||2005|
Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen binding activity.
MA5-13269 was used in EMSA to investigate the nucleophilic reactivity of natural antibodies
|Planque S,Taguchi H,Burr G,Bhatia G,Karle S,Zhou YX,Nishiyama Y,Paul S||The Journal of biological chemistry (278:20436)||2003|
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth f; epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; ERBB; ERBB1; HER1; mENA; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; waved 2
9030024J15Rik; AI552599; EGFR; ERBB; ERBB1; Errb1; Errp; HER1; mENA; NISBD2; PIG61; wa-2; wa2; Wa5