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Immunofluorescence analysis of EGFR was done on 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled EGFR (199.12) Mouse Monoclonal Antibody (MA513319) at 2ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Non-human primate, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Extracellular domain of human recombinant EGFR protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 7 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Blocking Assay (BLOCK)||See 2 publications below|
|Immunocytochemistry (ICC)||See 5 publications below|
|ELISA (ELISA)||See 2 publications below|
|Immunoprecipitation (IP)||See 17 publications below|
|Flow Cytometry (Flow)||See 3 publications below|
MA5-13319 targets Epidermal Growth Factor Receptor in IP, ICC/IF and WB applications and shows reactivity with Human samples.
The MA5-13319 immunogen is extracellular domain of human recombinant EGFR protein.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
IP-MS enrichment of EGFR (LFQ intensity): EGFR was enriched 54-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and EGFR antibody (Part No. MA5-13319). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Surfactant protein D suppresses lung cancer progression by downregulation of epidermal growth factor signaling.
MA5-13319 was used in immunoprecipitation and western blot to study the role of impaired EGF binding to EGFR in the mechanism by which surfactant protein D inhibits the progression of lung cancer
|Hasegawa Y,Takahashi M,Ariki S,Asakawa D,Tajiri M,Wada Y,Yamaguchi Y,Nishitani C,Takamiya R,Saito A,Uehara Y,Hashimoto J,Kurimura Y,Takahashi H,Kuroki Y||Oncogene (34:838)||2015|
High speed digital protein interaction analysis using microfluidic single molecule detection system.
MA5-13319 was used in western blot to study the use of a microfluidic single molecule detection system for the rapid analysis of protein interactions
|Chou CK,Jing N,Yamaguchi H,Tsou PH,Lee HH,Chen CT,Wang YN,Hong S,Su C,Kameoka J,Hung MC||Lab on a chip (10:1793)||2010|
EGFRvIII-induced estrogen-independence, tamoxifen-resistance phenotype correlates with PgR expression and modulation of apoptotic molecules in breast cancer.
MA5-13319 was used in western blot to study the ability of EGFRvIII to promote estrogen-independent growth in breast cancer cell lines
|Zhang Y,Su H,Rahimi M,Tochihara R,Tang C||International journal of cancer (125:2021)||2009|
EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2.
MA5-13319 was used in western blot to examine the role of EGFR and ADAMs in the endocytic trafficking of the desmosomal cadherin desmoglein 2
|Klessner JL,Desai BV,Amargo EV,Getsios S,Green KJ||Molecular biology of the cell (20:328)||2009|
Alix/AIP1 antagonizes epidermal growth factor receptor downregulation by the Cbl-SETA/CIN85 complex.
MA5-13319 was used in western blot to study the mechanism by which Alix/AIP1 antagonizes EGFR downregulation by the Cbl-SETA/CIN85 complex
|Schmidt MH,Hoeller D,Yu J,Furnari FB,Cavenee WK,Dikic I,Bögler O||Molecular and cellular biology (24:8981)||2004|
Epidermal growth factor receptor signaling intensity determines intracellular protein interactions, ubiquitination, and internalization.
MA5-13319 was used in western blot to study the role of EGFR signaling intensity in determining intracellular protein interactions, ubiquitination, and internalization
|Schmidt MH,Furnari FB,Cavenee WK,Bögler O||Proceedings of the National Academy of Sciences of the United States of America (100:6505)||2003|
A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera.
MA5-13319 was used in western blot to develop an acridium-linked immunosorbent assay for measuring soluble ErbB1 in human serum
|Baron AT,Lafky JM,Connolly DC,Peoples J,O'Kane DJ,Suman VJ,Boardman CH,Podratz KC,Maihle NJ||Journal of immunological methods (219:23)||1998|
mMAPS: a flow-proteometric technique to analyze protein-protein interactions in individual signaling complexes.
MA5-13319 was used in ChIP assay to develop a novel flow-proteomic methodology for the detection of individual signaling complex protein-protein interactions in crude cell/tissue lysates
|Chou CK,Lee HH,Tsou PH,Chen CT,Hsu JM,Yamaguchi H,Wang YN,Lee HJ,Hsu JL,Lee JF,Kameoka J,Hung MC||Science signaling (7:null)||2014|
Propranolol restricts the mobility of single EGF-receptors on the cell surface before their internalization.
MA5-13319 was used in blocking or activating experiment to study the ability of propanolol to restrict cell surface EGFR mobility prior to receptor internalization
|Otero C,Linke M,Sanchez P,González A,Schaap IA||PloS one (8:null)||2013|
Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays.
MA5-13319 was used in blocking or activating experiment to develop a bead-based sandwich immunoassay for the profiling of receptor tyrosine kinase inhibitors
|Pötz O,Schneiderhan-Marra N,Henzler T,Herget T,Joos TO||Methods in molecular biology (Clifton, N.J.) (795:191)||2011|
Internalization mechanisms of the epidermal growth factor receptor after activation with different ligands.
MA5-13319 was used in immunocytochemistry to study internalization of the EGFR following activation by various ligands and the different mechanisms involved
|Henriksen L,Grandal MV,Knudsen SL,van Deurs B,Grøvdal LM||PloS one (8:null)||2013|
Monitoring the size and lateral dynamics of ErbB1 enriched membrane domains through live cell plasmon coupling microscopy.
MA5-13319 was used in immunocytochemistry to study the dynamics of ErbB1 enriched membrane domains using live cell plasmon coupling microscopy
|Rong G,Reinhard BM||PloS one (7:null)||2012|
Spatial control of EGF receptor activation by reversible dimerization on living cells.
MA5-13319 was used in immunocytochemistry to investigate the effect of reversible EGF dimerization on its activation
|Chung I,Akita R,Vandlen R,Toomre D,Schlessinger J,Mellman I||Nature (464:783)||2010|
ARAP1 regulates endocytosis of EGFR.
MA5-13319 was used in immunocytochemistry to investigate the effect of ARAP1 on endocytosis of EGFR
|Yoon HY,Lee JS,Randazzo PA||Traffic (Copenhagen, Denmark) (9:2236)||2008|
The mucin Muc4 potentiates neuregulin signaling by increasing the cell-surface populations of ErbB2 and ErbB3.
MA5-13319 was used in immunocytochemistry to study the role of ErbB2 and ErbB3 in the mechanism by which Muc4 potentiates neuregulin signaling
|Funes M,Miller JK,Lai C,Carraway KL,Sweeney C||The Journal of biological chemistry (281:19310)||2006|
A Miniaturized Ligand Binding Assay for EGFR.
MA5-13319 was used in ELISA to develop a miniaturized ligand binding assay for EGFR
|Schwenk JM,Poetz O,Zeillinger R,Joos TO||International journal of proteomics (2012:null)||2012|
Profiling receptor tyrosine kinase activation by using Ab microarrays.
MA5-13319 was used in ELISA to study the receptor tyrosine kinase activation by through antibody microarrays
|Nielsen UB,Cardone MH,Sinskey AJ,MacBeath G,Sorger PK||Proceedings of the National Academy of Sciences of the United States of America (100:9330)||2003|
Superior antitumoral activity of dimerized targeted single-chain TRAIL fusion proteins under retention of tumor selectivity.
MA5-13319 was used in immunoprecipitation to develop targeted dimerized single-chain TRAIL fusion proteins displaying markedly increased anti-tumor activity
|Siegemund M,Pollak N,Seifert O,Wahl K,Hanak K,Vogel A,Nussler AK,Göttsch D,Münkel S,Bantel H,Kontermann RE,Pfizenmaier K||Cell death & disease (3:null)||2012|
IL-24 is expressed during wound repair and inhibits TGFalpha-induced migration and proliferation of keratinocytes.
MA5-13319 was used in immunoprecipitation to study the role of IL-24 in wound repair and its inhibitory effect on TGFalpha-induced migration and proliferation of keratinocytes
|Poindexter NJ,Williams RR,Powis G,Jen E,Caudle AS,Chada S,Grimm EA||Experimental dermatology (19:714)||2010|
Intracellular MUC1 peptides inhibit cancer progression.
MA5-13319 was used in immunoprecipitation to study the anti-tumor activity of cell-permeant peptides designed to block MUC1 interactions with beta-catenin and EGFR
|Bitler BG,Menzl I,Huerta CL,Sands B,Knowlton W,Chang A,Schroeder JA||Clinical cancer research : an official journal of the American Association for Cancer Research (15:100)||2009|
Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation.
MA5-13319 was used in immunoprecipitation to study the role of Ubc4/5 and c-Cbl in the polyubiquitination and degradation of internalized EGFR
|Umebayashi K,Stenmark H,Yoshimori T||Molecular biology of the cell (19:3454)||2008|
|Non-human primate||Not Cited||
Sensitivity of epidermal growth factor receptor and ErbB2 exon 20 insertion mutants to Hsp90 inhibition.
MA5-13319 was used in immunoprecipitation to study the sensitivity of EGFR and ErbB2 exon 20 insertion mutants to Hsp90 inhibition
|Xu W,Soga S,Beebe K,Lee MJ,Kim YS,Trepel J,Neckers L||British journal of cancer (97:741)||2007|
Acquired resistance to erlotinib in A-431 epidermoid cancer cells requires down-regulation of MMAC1/PTEN and up-regulation of phosphorylated Akt.
MA5-13319 was used in immunoprecipitation to study the mechanism of acquired resistance to erlotinib in epidermoid cancer cells
|Yamasaki F,Johansen MJ,Zhang D,Krishnamurthy S,Felix E,Bartholomeusz C,Aguilar RJ,Kurisu K,Mills GB,Hortobagyi GN,Ueno NT||Cancer research (67:5779)||2007|
Intersectin regulates epidermal growth factor receptor endocytosis, ubiquitylation, and signaling.
MA5-13319 was used in immunoprecipitation to study the mechanism by which the endocytic scaffold protein intersectin regulates EGFR degradation and signaling
|Martin NP,Mohney RP,Dunn S,Das M,Scappini E,O'Bryan JP||Molecular pharmacology (70:1643)||2006|
Diacylglycerol kinase delta regulates protein kinase C and epidermal growth factor receptor signaling.
MA5-13319 was used in immunoprecipitation to study the role of PKC in the mechanism by which diacylglycerol kinase-delta regulates EGFR signaling
|Crotty T,Cai J,Sakane F,Taketomi A,Prescott SM,Topham MK||Proceedings of the National Academy of Sciences of the United States of America (103:15485)||2006|
Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1.
MA5-13319 was used in immunoprecipitation to study the mechanism of EGFR nuclear-cytoplasmic transport
|Lo HW,Ali-Seyed M,Wu Y,Bartholomeusz G,Hsu SC,Hung MC||Journal of cellular biochemistry (98:1570)||2006|
Geldanamycin-associated inhibition of intracellular trafficking is attributed to a co-purified activity.
MA5-13319 was used in immunoprecipitation to study a co-purifying contaminant of geldanamycin preparations as the component inhibiting intracellular trafficking
|Barzilay E,Ben-Califa N,Supino-Rosin L,Kashman Y,Hirschberg K,Elazar Z,Neumann D||The Journal of biological chemistry (279:6847)||2004|
1,25-Dihydroxyvitamin D down-regulates cell membrane growth- and nuclear growth-promoting signals by the epidermal growth factor receptor.
MA5-13319 was used in immunoprecipitation to study the down regulatory effects of 1,25-dihydroxyvitamin D on EGFR membrane trafficing and growth signaling
|Cordero JB,Cozzolino M,Lu Y,Vidal M,Slatopolsky E,Stahl PD,Barbieri MA,Dusso A||The Journal of biological chemistry (277:38965)||2002|
Metalloproteinases stimulate ErbB-dependent ERK signaling in human skin organ culture.
MA5-13319 was used in immunoprecipitation to study the role of MMPs in stimulating ErbB-dependent ERK signaling in human skin organ culture
|Stoll SW,Kansra S,Elder JT||The Journal of biological chemistry (277:26839)||2002|
Epidermal growth factor receptor signaling and the invasive phenotype of ovarian carcinoma cells.
MA5-13319 was used in immunoprecipitation to study the role of EGFR signaling in the invasiveness of ovarian carcinoma cells
|Bergmann-Leitner ES,Bennett TA,Hacker NF,Stromberg K,Stetler-Stevenson WG||Journal of the National Cancer Institute (93:1375)||2001|
Tyrosine-phosphorylated plakoglobin is associated with desmogleins but not desmoplakin after epidermal growth factor receptor activation.
MA5-13319 was used in immunoprecipitation to study the effects of EGFR activation on the association of tyrosine phosphorylated plakoglobin with desmogleins and desmoplakin
|Gaudry CA,Palka HL,Dusek RL,Huen AC,Khandekar MJ,Hudson LG,Green KJ||The Journal of biological chemistry (276:24871)||2001|
Intracellular retention and degradation of the epidermal growth factor receptor, two distinct processes mediated by benzoquinone ansamycins.
MA5-13319 was used in immunoprecipitation to study the effect of the benzoquinone ansamycins on the intracellular retention and degradation of EGFR
|Supino-Rosin L,Yoshimura A,Yarden Y,Elazar Z,Neumann D||The Journal of biological chemistry (275:21850)||2000|
Epidermal growth factor receptor vIII enhances tumorigenicity in human breast cancer.
MA5-13319 was used in immunoprecipitation to study the role of the constitutively active EGFR vIII variant in the proliferation and tumorigenicity of human breast cancer
|Tang CK,Gong XQ,Moscatello DK,Wong AJ,Lippman ME||Cancer research (60:3081)||2000|
A cytosolic domain of the erythropoietin receptor contributes to endoplasmic reticulum-associated degradation.
MA5-13319 was used in immunoprecipitation to study the role of the cytosolic domain of the EPO-R in endoplasmic reticulum-associated degradation
|Supino-Rosin L,Yoshimura A,Altaratz H,Neumann D||European journal of biochemistry / FEBS (263:410)||1999|
Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast.
MA5-13319 was used in flow cytometry to express functional and properly folded EGFR extracellular domain in yeast
|Kim YS,Bhandari R,Cochran JR,Kuriyan J,Wittrup KD||Proteins (62:1026)||2006|
Functional effects of glycosylation at Asn-579 of the epidermal growth factor receptor.
MA5-13319 was used in flow cytometry to study the functional effects of N-glycosylation of Asn-579 of EGFR
|Whitson KB,Whitson SR,Red-Brewer ML,McCoy AJ,Vitali AA,Walker F,Johns TG,Beth AH,Staros JV||Biochemistry (44:14920)||2005|
Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments.
MA5-13319 was used in flow cytometry to study the use of yeast surface display for identifying stable folded protein domains and characterizing antibody binding epitopes
|Cochran JR,Kim YS,Olsen MJ,Bhandari R,Wittrup KD||Journal of immunological methods (287:147)||2004|
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth f; erb-b2 receptor tyrosine kinase 1; ERBB; ERBB1; HER1; mENA; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61