Immunofluorescent analysis of Phalloidin (orange) and EGFR (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an EGFR monoclonal antibody (Product # MA5-12880) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified EGFR from A431 cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunoprecipitation (IP)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 7 publications below|
|Blocking Assay (BLOCK)||See 9 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Western Blot (WB)||See 3 publications below|
|Flow Cytometry (Flow)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Gel Shift (GS)||See 1 publications below|
MA5-12880 targets Epidermal Growth Factor Receptor in IP and IF applications and shows reactivity with Human and Non-human primate samples.
The MA5-12880 immunogen is purified EGFR from A431 cells.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
IP-MS enrichment of EGFR (LFQ intensity): EGFR was enriched 23-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and EGFR antibody (Part No. MA5-12880). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Interaction of EGFR to ¿-catenin leads to ¿-catenin phosphorylation and enhances EGFR signaling.
MA5-12880 was used in immunocytochemistry to study the interaction between EGFR and delta catenin, which leads to delta catenin phosphorylation and EGFR signaling enhancement
|He Y,Ryu T,Shrestha N,Yuan T,Kim H,Shrestha H,Cho YC,Seo YW,Song WK,Kim K||Scientific reports (6:null)||2016|
In vitro perforation of human epithelial carcinoma cell with antibody-conjugated biodegradable microspheres illuminated by a single 80 femtosecond near-infrared laser pulse.
MA5-12880 was used in immunocytochemistry to study the ability of antibody-conjugated biodegradable microspheres excited by a single 80 femtosecond near-IR laser pulse to perforate the cell membrane of epithelial carcinoma cells
|Terakawa M,Tsunoi Y,Mitsuhashi T||International journal of nanomedicine (7:2653)||2012|
The ß-catenin/Tcf4/survivin signaling maintains a less differentiated phenotype and high proliferative capacity of human corneal epithelial progenitor cells.
MA5-12880 was used in immunocytochemistry to study the role of the beta-catenin-Tcf4-survivin signaling pathway in human corneal epithelial progenitor cells
|Lu R,Bian F,Zhang X,Qi H,Chuang EY,Pflugfelder SC,Li DQ||The international journal of biochemistry and cell biology (43:751)||2011|
Nano-bio-chip sensor platform for examination of oral exfoliative cytology.
MA5-12880 was used in immunocytochemistry to develop a nano-bio-chip sensor to detect biomarkers of early oral tumorigenesis
|Weigum SE,Floriano PN,Redding SW,Yeh CK,Westbrook SD,McGuff HS,Lin A,Miller FR,Villarreal F,Rowan SD,Vigneswaran N,Williams MD,McDevitt JT||Cancer prevention research (Philadelphia, Pa.) (3:518)||2010|
The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells.
MA5-12880 was used in immunocytochemistry to study the role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells
|Ward DM,Vaughn MB,Shiflett SL,White PL,Pollock AL,Hill J,Schnegelberger R,Sundquist WI,Kaplan J||The Journal of biological chemistry (280:10548)||2005|
Interaction between tyrosine kinase Etk and a RUN domain- and FYVE domain-containing protein RUFY1. A possible role of ETK in regulation of vesicle trafficking.
MA5-12880 was used in immunocytochemistry to study the interaction between tyrosine kinase Etk and RUFY1 and the potential role in vesicle trafficking
|Yang J,Kim O,Wu J,Qiu Y||The Journal of biological chemistry (277:30219)||2002|
Differential utilization and localization of ErbB receptor tyrosine kinases in skin compared to normal and malignant keratinocytes.
MA5-12880 was used in immunocytochemistry and immunoprecipitation to study the differential utilization and localization of ErbB receptor tyrosine kinases in skin compared to normal and malignant keratinocytes
|Stoll SW,Kansra S,Peshick S,Fry DW,Leopold WR,Wiesen JF,Sibilia M,Zhang T,Werb Z,Derynck R,Wagner EF,Elder JT||Neoplasia (New York, N.Y.) (3:339)||2001|
PHD4 stimulates tumor angiogenesis in osteosarcoma cells via TGF-¿.
MA5-12880 was used in blocking or activating experiment to study the role of TGF-alpha in the mechanism by which prolyl hydroxylase-4 promotes osteosarcoma cell tumor angiogenesis
|Klotzsche-von Ameln A,Prade I,Grosser M,Kettelhake A,Rezaei M,Chavakis T,Flamme I,Wielockx B,Breier G||Molecular cancer research : MCR (11:1337)||2013|
The antimicrobial peptide LL37 induces the migration of human pulp cells: a possible adjunct for regenerative endodontics.
MA5-12880 was used in blocking or activating experiment to investigate the influence of antimicrobial peptide LL37 on human pulp cell migration
|Kajiya M,Shiba H,Komatsuzawa H,Ouhara K,Fujita T,Takeda K,Uchida Y,Mizuno N,Kawaguchi H,Kurihara H||Journal of endodontics (36:1009)||2010|
An EGFR autocrine loop encodes a slow-reacting but dominant mode of mechanotransduction in a polarized epithelium.
MA5-12880 was used in blocking or activating experiment to investigate lateral intercellular space deformation between bronchial epithelial cells in response to compressive stress
|Kojic N,Chung E,Kho AT,Park JA,Huang A,So PT,Tschumperlin DJ||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (24:1604)||2010|
Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor.
MA5-12880 was used in blocking or activating experiment to study the role of HB-EGF as a survival factor in human cytotrophoblast cells exposed to hypoxia/reoxygenation injury
|Leach RE,Kilburn BA,Petkova A,Romero R,Armant DR||American journal of obstetrics and gynecology (198:471.e1)||2008|
Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
MA5-12880 was used in blocking or activating experiment to examine the anti-apoptotic effect of epidermal growth factor-like growth factors in human placental cytotrophoblast cells
|Wolff GS,Chiang PJ,Smith SM,Romero R,Armant DR||Biology of reproduction (77:53)||2007|
Inhibition of xenograft tumor growth and down-regulation of ErbB receptors by an antibody directed against Lewis Y antigen.
MA5-12880 was used in blocking or activating experiment to study the inhibition of xenograft tumor growth and down-regulation of ErbB receptors by an antibody directed against Lewis Y antigen
|Farhan H,Schuster C,Klinger M,Weisz E,Waxenecker G,Schuster M,Sexl V,Mudde GC,Freissmuth M,Kircheis R||The Journal of pharmacology and experimental therapeutics (319:1459)||2006|
Receptor-selective retinoids inhibit the growth of normal and malignant breast cells by inducing G1 cell cycle blockade.
MA5-12880 was used in blocking or activating experiment to study the role of G1 cell cycle blockade in the inhibition of normal and cancerous breast cell gowth by synthetic, receptor selctive retionoids
|Wu K,DuPré E,Kim H,Tin-U CK,Bissonnette RP,Lamph WW,Brown PH||Breast cancer research and treatment (96:147)||2006|
Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
MA5-12880 was used in blocking or activating experiment to study the role of metalloproteinase-mediated shedding of HB-EGF-like growth factor in human trophoblast survival at low oxygen concentrations
|Armant DR,Kilburn BA,Petkova A,Edwin SS,Duniec-Dmuchowski ZM,Edwards HJ,Romero R,Leach RE||Development (Cambridge, England) (133:751)||2006|
Heparin-binding epidermal growth factor-like growth factor: hypoxia-inducible expression in vitro and stimulation of neurogenesis in vitro and in vivo.
MA5-12880 was used in blocking or activating experiment to study the possible role of HB-EGF in hypoxic-ischemic induction of neurogenesis
|Jin K,Mao XO,Sun Y,Xie L,Jin L,Nishi E,Klagsbrun M,Greenberg DA||The Journal of neuroscience : the official journal of the Society for Neuroscience (22:5365)||2002|
Epidermal growth factor receptor as a biomarker for cervical cancer.
MA5-12880 was used in immunohistochemistry to investigate the expression of epidermal growth factor receptor in cervical cancer
|Soonthornthum T,Arias-Pulido H,Joste N,Lomo L,Muller C,Rutledge T,Verschraegen C||Annals of oncology : official journal of the European Society for Medical Oncology (22:2166)||2011|
Involvement of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of epidermal growth factor receptor and mitogen-activated protein kinases in gastric carcinoma cells.
MA5-12880 was used in western blot to investigate the role of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of EGFR and MAPK in gastric carcinoma cells
|Yasuda H,Hirata S,Inoue K,Mashima H,Ohnishi H,Yoshiba M||Biochemical and biophysical research communications (354:154)||2007|
Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.
MA5-12880 was used in western blot to investigate the mechanism of ERK autocrine activation in normal human keratinocytes
|Kansra S,Stoll SW,Johnson JL,Elder JT||Molecular biology of the cell (15:4299)||2004|
Autoantibodies to the epidermal growth factor receptor in systemic sclerosis, lupus, and autoimmune mice.
MA5-12880 was used in western blot to study the auto-immune response against epidermal growth factor receptor
|Planque S,Zhou YX,Nishiyama Y,Sinha M,O'Connor-Mccourt M,Arnett FC,Paul S||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (17:136)||2003|
Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast.
MA5-12880 was used in flow cytometry to express functional and properly folded EGFR extracellular domain in yeast
|Kim YS,Bhandari R,Cochran JR,Kuriyan J,Wittrup KD||Proteins (62:1026)||2006|
Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments.
MA5-12880 was used in flow cytometry to study the use of yeast surface display for identifying stable folded protein domains and characterizing antibody binding epitopes
|Cochran JR,Kim YS,Olsen MJ,Bhandari R,Wittrup KD||Journal of immunological methods (287:147)||2004|
Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display.
MA5-12880 was used in immunoprecipitation to map the epitopes of therapeutically important epidermal growth factor receptor antibodies
|Chao G,Cochran JR,Wittrup KD||Journal of molecular biology (342:539)||2004|
Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen binding activity.
MA5-12880 was used in EMSA to investigate the nucleophilic reactivity of natural antibodies
|Planque S,Taguchi H,Burr G,Bhatia G,Karle S,Zhou YX,Nishiyama Y,Paul S||The Journal of biological chemistry (278:20436)||2003|