|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/mg lysate|
|Western Blot (WB)||0.5-1 µg/ml|
|Immunocytochemistry (ICC)||See 4 publications below|
|Neutralization (Neu)||See 2 publications below|
|Western Blot (WB)||See 28 publications below|
|Immunoprecipitation (IP)||See 3 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Flow Cytometry (Flow)||See 3 publications below|
|ELISA (ELISA)||See 2 publications below|
|Gel Shift (GS)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||HC2 20 d2 cells.|
|Storage buffer||PBS, pH 7.4|
MA1-12693 detects Epidermal Growth Factor Receptor in human samples.
MA1-12693 has been successfully used in FACS, immunofluorescence, immunoprecipitation, Western blot, and immunohistochemistry (paraffin) procedures. Staining of formalin-fixed tissues requires digestion with protease xxv at 1 mg/ml in PBS for 5 minutes at 37°C. Positive controls include A431 cells or placenta or squamous cell carcinoma. Suitable for Western blotting of extracellular domain of EGFR.
The MA1-12693 immunogen is HC2 20 d2 cells.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth f; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; ERBB; ERBB1; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; waved 2
Gene Aliases: 9030024J15Rik; AI552599; EGFR; ERBB; ERBB1; Errb1; Errp; HER1; mENA; NISBD2; PIG61; wa-2; wa2; Wa5
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