|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from C-terminus of human EGFR protein.|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta or lung tissue.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hypoxia activated EGFR signaling induces epithelial to mesenchymal transition (EMT).
MA5-16359 was used in western blot to study the induction of epithelial-to-mesenchymal transition by hypoxia-induced EGFR signaling and the significance for metastasis
|Misra A,Pandey C,Sze SK,Thanabalu T||PloS one (7:null)||2012|
Decorin is a part of the ovarian extracellular matrix in primates and may act as a signaling molecule.
MA5-16359 was used in western blot to study the presence of decorin in the primate ovarian extracellular matrix and its potential signaling role
|Adam M,Saller S,Ströbl S,Hennebold JD,Dissen GA,Ojeda SR,Stouffer RL,Berg D,Berg U,Mayerhofer A||Human reproduction (Oxford, England) (27:3249)||2012|