|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Extracellular domain of human recombinant EGFR protein.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Antigen retrieval is recommended prior to staining, using Protease XXV at 1mg/ml in PBS for 5 minutes at 37°C. Incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, placenta or squamous cell carcinoma.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hypoxia activated EGFR signaling induces epithelial to mesenchymal transition (EMT).
MA1-37605 was used in western blot to study the induction of epithelial-to-mesenchymal transition by hypoxia-induced EGFR signaling and the significance for metastasis
|Misra A,Pandey C,Sze SK,Thanabalu T||PloS one (7:null)||2012|
Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Cell surface receptor mediated signal transduction Receptor protein tyrosine kinase signaling pathway Oncogenesis Developmental processes Gametogenesis Oogenesis Embryogenesis Determination of dorsal/ventral axis Cell cycle Cell cycle control Apoptosis Inhibition of apoptosis Cell proliferation and differentiation