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Immunofluorescent analysis of EGFR (green) showing staining in the cytoplasm and membrane of A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Epidermal Growth Factor Receptor monoclonal antibody (Product # AHR5072) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||The extracellular domain of human recombinant EGFR protein, purified.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
AHR5072 reacts with an extracellular domain of EGFR. The molecular weight of the antigen is 170kDa (wild type) and 145kDa (vIII variant). This antibody does not block the binding of EGF to EGFR.
Epidermal Growth Factor Receptor (EGFR) is a 175 kDa transmembrane glycoprotein belonging to tyrosine kinase superfamily. It acts as a receptor for epidermal growth factor (EGF) family proteins. Binding of EGFR to its ligands causes autophosphorylation of tyrosine kinase followed by activation of signal transduction pathways connected to cell proliferation and differentiation. Tyrosine 1068 present within the cytoplasmic domain of the receptor is a major autophosphorylation site that allows binding of Grb2 and activation of the Ras-Raf-ERK1 and ERK2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 22.214.171.124; Epidermal growth factor receptor precursor; erb-b2 receptor tyrosine kinase 1; ERBB1; kinase EGFR; proto-oncogene c-ErbB-1; Receptor protein-tyrosine kinase ErbB-1; receptor tyrosine-protein kinase erbB-1
EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61