Western blot analysis was performed on whole cell extracts (30 ug lysate) of A431 (Lane 1), A549 (Lane 2) and HeLa (Lane 3). The blots were probed with Anti-EGFR Mouse Monoclonal Antibody (Product# MA513877, 1-3 ug/ml) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 626520, 1:4000 dilution). A 180 kDa band corresponding to EGFR was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with by overnight wet transfer method. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human|
|Published species reactivity||Fruit fly, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified EGFR from A431 cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13877 targets Epidermal Growth Factor Receptor in IP and WB applications and shows reactivity with Human samples.
The MA5-13877 immunogen is purified EGFR from A431 cells.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EGFR blockade enriches for lung cancer stem-like cells through Notch3-dependent signaling.
MA5-13877 was used in immunoprecipitation and western blot to examine the effects of kinase inhibtors that target the epidermal growth factor receptor in lung cancer
|Arasada RR,Amann JM,Rahman MA,Huppert SS,Carbone DP||Cancer research (74:5572)||2014|
Evaluation of the antitumor activity of dacomitinib in models of human bladder cancer.
MA5-13877 was used in western blot to study the efficacy and tolerability of the irreversible tyrosine kinase inhibitor dacomitinib in human bladder cancer models
|Grivas PD,Day KC,Karatsinides A,Paul A,Shakir N,Owainati I,Liebert M,Kunju LP,Thomas D,Hussain M,Day ML||Molecular medicine (Cambridge, Mass.) (19:367)||2013|
Modulating therapeutic effects of the c-Src inhibitor via oestrogen receptor and human epidermal growth factor receptor 2 in breast cancer cell lines.
MA5-13877 was used in western blot to study the influence of estrogen receptor and HER2 expression on the therapeutic efficacy of a c-Src inhibitor in a panel of breast cancer cell lines
|Fan P,McDaniel RE,Kim HR,Clagett D,Haddad B,Jordan VC||European journal of cancer (Oxford, England : 1990) (48:3488)||2012|
Pregnancy-upregulated nonubiquitous calmodulin kinase induces ligand-independent EGFR degradation.
MA5-13877 was used in western blot to study the induction of ligand-independent EGFR degradation by the pregnancy-upregulated non-ubiquitous calmodulin kinase
|Deb TB,Coticchia CM,Barndt R,Zuo H,Dickson RB,Johnson MD||American journal of physiology. Cell physiology (295:C365)||2008|
Targeting EGFR in bladder cancer.
MA5-13877 was used in western blot to study EGFR expression in bladder cancer
|Villares GJ,Zigler M,Blehm K,Bogdan C,McConkey D,Colin D,Bar-Eli M||World journal of urology (25:573)||2007|
EGF-independent activation of cell-surface EGF receptors harboring mutations found in gefitinib-sensitive lung cancer.
MA5-13877 was used in western blot to study EGF-independent activation of mutant cell-surface EGF receptors in gefitinib-sensitive lung cancer
|Choi SH,Mendrola JM,Lemmon MA||Oncogene (26:1567)||2007|
Gangliosides play an important role in the organization of CD82-enriched microdomains.
MA5-13877 was used in western blot to study the role of gangliosides in membrane micro-domains enriched in the tetraspanin CD82
|Odintsova E,Butters TD,Monti E,Sprong H,van Meer G,Berditchevski F||The Biochemical journal (400:315)||2006|
Microarray analyses in bladder cancer cells: inhibition of hTERT expression down-regulates EGFR.
MA5-13877 was used in western blot to study the antiproliferative capacity of hTERT antisense oligonucleotides
|Kraemer K,Schmidt U,Fuessel S,Herr A,Wirth MP,Meye A||International journal of cancer (119:1276)||2006|
Mutations within the kinase domain and truncations of the epidermal growth factor receptor are rare events in bladder cancer: implications for therapy.
MA5-13877 was used in western blot to study the therapeutic implications of mutations and truncations of the EGF receptor in bladder cancer
|Blehm KN,Spiess PE,Bondaruk JE,Dujka ME,Villares GJ,Zhao YJ,Bogler O,Aldape KD,Grossman HB,Adam L,McConkey DJ,Czerniak BA,Dinney CP,Bar-Eli M||Clinical cancer research : an official journal of the American Association for Cancer Research (12:4671)||2006|
ErbB receptor tyrosine kinase network inhibition radiosensitizes carcinoma cells.
MA5-13877 was used in western blot to study the radiosensitization of carcinoma cells by inhibition of the ErbB receptor tyrosine kinase network by the EGFR-CD533 truncation mutant
|Contessa JN,Abell A,Valerie K,Lin PS,Schmidt-Ullrich RK||International journal of radiation oncology, biology, physics (65:851)||2006|
|Fruit fly||Not Cited||
Epidermal growth factor receptor dimerization and activation require ligand-induced conformational changes in the dimer interface.
MA5-13877 was used in western blot to study the role of ligand-induced dimer interface conformational changes in the dimerization and activation of EGFR
|Dawson JP,Berger MB,Lin CC,Schlessinger J,Lemmon MA,Ferguson KM||Molecular and cellular biology (25:7734)||2005|
Src family kinase inhibitors block amphiregulin-mediated autocrine ErbB signaling in normal human keratinocytes.
MA5-13877 was used in western blot to study the interplay between Src family kinases and ErbB signaling in normal human keratinocytes
|Kansra S,Stoll SW,Johnson JL,Elder JT||Molecular pharmacology (67:1145)||2005|
Activated EGFR stimulates MUC1 expression in human uterine and pancreatic cancer cell lines.
MA5-13877 was used in western blot to examine the role that EGFR activation plays in modulating MUC1 levels.
|Engel BJ,Carson DD||Journal of cellular biochemistry (114:2314)||2013|
The MUC1 Ectodomain: A Novel and Efficient Target for Gold Nanoparticle Clustering and Vapor Nanobubble Generation.
MA5-13877 was used in western blot to assess EGFR and MUC1 as targets for nanoparticle intracellular accumulation and clustering.
|Danysh BP,Constantinou PE,Lukianova-Hleb EY,Lapotko DO,Carson DD||Theranostics (2:777)||2012|
Metastasis suppressor tetraspanin CD82/KAI1 regulates ubiquitylation of epidermal growth factor receptor.
MA5-13877 was used in immunocytochemistry and western blot to study the modulation of EGFR ubiquitinylation by the CD82 metastasis suppressor
|Odintsova E,van Niel G,Conjeaud H,Raposo G,Iwamoto R,Mekada E,Berditchevski F||The Journal of biological chemistry (288:26323)||2013|
A novel proximity assay for the detection of proteins and protein complexes: quantitation of HER1 and HER2 total protein expression and homodimerization in formalin-fixed, paraffin-embedded cell lines and breast cancer tissue.
MA5-13877 was used in immunocytochemistry to develop a novel proximity assay for detecting HER proteins and homodimers in formalin-fixed cell lines and breast tumor tissues
|Shi Y,Huang W,Tan Y,Jin X,Dua R,Penuel E,Mukherjee A,Sperinde J,Pannu H,Chenna A,DeFazio-Eli L,Pidaparthi S,Badal Y,Wallweber G,Chen L,Williams S,Tahir H,Larson J,Goodman L,Whitcomb J,Petropoulos C,Winslow J||Diagnostic molecular pathology : the American journal of surgical pathology, part B (18:11)||2009|
Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action.
MA5-13877 was used in immunoprecipitation to investigate the state of HER1-HER2 interaction and modification in cell lines and tumors
|DeFazio-Eli L,Strommen K,Dao-Pick T,Parry G,Goodman L,Winslow J||Breast cancer research : BCR (13:null)||2011|
Compensatory ErbB3/c-Src signaling enhances carcinoma cell survival to ionizing radiation.
MA5-13877 was used in immunoprecipitation to study a novel ErbB3/c-Src signaling pathway that protects carcinoma cells against radiation-induced apoptosis
|Contessa JN,Abell A,Mikkelsen RB,Valerie K,Schmidt-Ullrich RK||Breast cancer research and treatment (95:17)||2006|
Requirement of Tyr-992 and Tyr-1173 in phosphorylation of the epidermal growth factor receptor by ionizing radiation and modulation by SHP2.
MA5-13877 was used in immunoprecipitation to study the role of phosphorylation of tyrosines 992 and 1173 in the activation of EGFR by ionizing radiation and the involvement of SHP2
|Sturla LM,Amorino G,Alexander MS,Mikkelsen RB,Valerie K,Schmidt-Ullrichr RK||The Journal of biological chemistry (280:14597)||2005|
Quantitative role of the human papillomavirus type 16 E5 gene during the productive stage of the viral life cycle.
MA5-13877 was used in immunoprecipitation and western blot to study the role of the HPV 16 E5 gene during the productive stage of the viral life cycle
|Genther SM,Sterling S,Duensing S,Münger K,Sattler C,Lambert PF||Journal of virology (77:2832)||2003|