Invitrogen

EGR1 Monoclonal Antibody (T.160.5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

8 References

View all (24) EGR1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

EGR1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of EGR1 in dissociated PC12 cells using an EGR1 monoclonal antibody (Product # MA5-15008) (green) and a MEK1/2 monoclonal antibody (red).

EGR1 Antibody in Western Blot (WB) — Western blot analysis was performed on modified whole cell extracts (1% SDS) of PC-12 (Lane 1), PC-12 Serum Starved (Serum starved for overnight) (Lane 2), PC-12 Serum Starved and then treated with NGF (Serum Starved for overnight, NGF treatment at 100ng/ml for 2hr) (Lane 3), NIH/3T3 (Lane 4), SW480 (Lane 5), A-431 (Lane 6), HeLa (Lane 7) and A-375 (Lane 8). The blot was probed with Anti-EGR1 Monoclonal Antibody (Product # MA5-15008, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 56 kDa band corresponding to EGR1 was detected across the cell lines and tissues tested. Also there was an induction of EGR1 in PC-12 cells upon NGF treatment.

EGR1 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 5 m 6 A promotes the translation of immediate-early genes upon activity induction. a Immediate-early genes (IEGs) are comparably induced by fear conditioning training in the hippocampus of both the CTRL (blue) and cKO (red) mice. b Increment of m 6 A modification abundance on IEG mRNAs after fear conditioning training in hippocampus samples of the CTRL mice but not the Mettl3 cKO mice. IP, immunoprecipitated fraction. c Translation of IEGs is impaired in the cKO mice after training. d Immunofluorescent image of EGR1 in CA1 region before and after fear conditioning training (1 h and 4 h). e Translation of IEGs is impaired in Mettl3 -KO primary cortical neurons, which can be rescued by re-expression of wildtype Mettl3 but not mutated Mettl3 . Student's t -test, * P < 0.05, ** P < 0.01, *** P < 0.001, N.S., not significant, ( a ) n = 2 replicates, ( b ), ( c ) and ( e ) n = 3 replicates

EGR1 Antibody in Western Blot, Immunohistochemistry (PFA fixed) (WB, IHC (PFA)) — FIGURE 8 Expression of 35 key genes and EGR1 in AKI samples and sham samples. (A) Transcriptional levels of 35 important genes including 21 hub genes and 14 other important upregulated genes in our own AKI samples. (B) Translational levels of Egr1 in AKI samples and sham samples. (C) Western blot of EGR1. (D,E) Immunofluorescence of EGR1. * p < 0.05, ** p < 0.01, *** p < 0.001.

EGR1 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using EGR1 Monoclonal Antibody (Product # MA5-15008), shows increased expression of proteins in PC-12 cells treated with NGF.

Product Details

MA5-15008

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,600

View 1 publication 1 publication

Immunoprecipitation (IP)

1:50

ChIP assay (ChIP)

1:50

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

T.160.5

Immunogen

Synthetic peptide corresponding to the amino terminus of the sequence of human EGR1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

42 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10977090

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Differential Gain-of-Function Activity of Three p53 Hotspot Mutants In Vivo.

Cancer research

Xiong S,Chachad D,Zhang Y,Gencel-Augusto J,Sirito M,Pant V,Yang P,Sun C,Chau G,Qi Y,Su X,Whitley EM,El-Naggar AK,Lozano G

MA5-15008 was used in Western Blotting to show that p53 hotspot mutants inhibit wild-type p53 similarly but differ in their GOF activities, with stronger tumor-promoting activity in contact mutants and distinct protein partners of each mutant driving tumorigenesis and metastasis.

Mon May 16 00:00:00 EDT 2022

Identification and Validation of Potential Biomarkers and Their Functions in Acute Kidney Injury.

Frontiers in genetics

Chen J,Chen Y,Olivero A,Chen X

MA5-15008 was used in Western Blot, Immunocytochemistry to investigate crucial genes correlated with AKI, and their potential functions, which might contribute to a better understanding of AKI pathogenesis.

Mon Sep 28 00:00:00 EDT 2020

Identification and Validation of Potential Biomarkers and Their Functions in Acute Kidney Injury.

Frontiers in genetics

Chen J,Chen Y,Olivero A,Chen X

Mon Sep 28 00:00:00 EDT 2020

Identification and Validation of Potential Biomarkers and Their Functions in Acute Kidney Injury.

Frontiers in genetics

Chen J,Chen Y,Olivero A,Chen X

Mon Sep 28 00:00:00 EDT 2020

Identification and Validation of Potential Biomarkers and Their Functions in Acute Kidney Injury.

Frontiers in genetics

Chen J,Chen Y,Olivero A,Chen X

Mon Sep 28 00:00:00 EDT 2020

Fasting-mimicking diet and hormone therapy induce breast cancer regression.

Nature

Caffa I,Spagnolo V,Vernieri C,Valdemarin F,Becherini P,Wei M,Brandhorst S,Zucal C,Driehuis E,Ferrando L,Piacente F,Tagliafico A,Cilli M,Mastracci L,Vellone VG,Piazza S,Cremonini AL,Gradaschi R,Mantero C,Passalacqua M,Ballestrero A,Zoppoli G,Cea M,Arrighi A,Odetti P,Monacelli F,Salvadori G,Cortellino S,Clevers H,De Braud F,Sukkar SG,Provenzani A,Longo VD,Nencioni A

MA5-15008 was used in Western Blotting to support further clinical studies of a fasting-mimicking diet as an adjuvant to oestrogen therapy in hormone-receptor-positive breast cancer.

Wed Jul 01 00:00:00 EDT 2020

METTL3-mediated N⁶-methyladenosine mRNA modification enhances long-term memory consolidation.

Cell research

Zhang Z,Wang M,Xie D,Huang Z,Zhang L,Yang Y,Ma D,Li W,Zhou Q,Yang YG,Wang XJ

Published figure using EGR1 monoclonal antibody (Product # MA5-15008) in Immunofluorescence

Thu Nov 01 00:00:00 EDT 2018

METTL3-mediated N⁶-methyladenosine mRNA modification enhances long-term memory consolidation.

Cell research

Zhang Z,Wang M,Xie D,Huang Z,Zhang L,Yang Y,Ma D,Li W,Zhou Q,Yang YG,Wang XJ

Published figure using EGR1 monoclonal antibody (Product # MA5-15008) in Immunofluorescence

Thu Nov 01 00:00:00 EDT 2018

Melatonin attenuates scopolamine-induced memory/synaptic disorder by rescuing EPACs/miR-124/Egr1 pathway.

Molecular neurobiology

Wang X,Wang ZH,Wu YY,Tang H,Tan L,Wang X,Gao XY,Xiong YS,Liu D,Wang JZ,Zhu LQ

MA5-15008 was used in western blot to study the molecular mechanisms by which melatonin treatment improves memory in a scopolamine-induced amnesia model

Fri Feb 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: AT225; Early growth response protein 1; EGR-1; Krox-24 nuclear protein; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24

Gene Aliases: A530045N19Rik; AT225; egr; Egr-1; EGR1; ETR103; G0S30; Krox-1; KROX-24; KROX24; Ngf1; NGF1-A; Ngfi; NGFI-A; NGFIA; TIS8; Zenk; Zfp-6; ZIF-268; Zif268; ZNF225

UniProt ID: (Human) P18146, (Rat) P08154, (Mouse) P08046

Entrez Gene ID: (Human) 1958, (Rat) 24330, (Mouse) 13653

Molecular Function: C2H2 zinc finger transcription factor