|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 518-563 of human EHHADH|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 79 kDa.
Purity is >95% by SDS-PAGE.
As an L-hydroxy-specific enzyme, EHHADH (enoyl-CoA-hydratase:3-hydroxyacyl-CoA dehydrogenase), also known as Peroxisomal L-bifunctional enzyme, is a 723 amino acid protein has an essential tripeptide sequence on its carboxyl-terminus that is required for peroxisomal transport. EHHADH-null mice only exhibit a blunted peroxisome proliferative response when challenged with a peroxisome proliferator. Since there were no observed changes in lipid metabolism, this evidence suggests that enoyl-CoAs were diverted to the D-hydroxy-specific beta-oxidation system for metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.