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Immunofluorescent analysis of EIF2 beta showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an EIF2 beta polyclonal antibody (Product # PA5-28469) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 119 and 331 of Human EIF2 beta|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28469 targets EIF2 beta in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-28469 immunogen is recombinant fragment corresponding to a region within amino acids 119 and 331 of Human EIF2 beta.
Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
eIF-2-beta; EIF2; EIF2B; EIF2beta; eukaryotic initiation factor 2-beta; eukaryotic translation initiation factor 2 beta; eukaryotic translation initiation factor 2 subunit 2; eukaryotic translation initiation factor 2 subunit beta; eukaryotic translation initiation factor 2, subunit 2 (beta, 38kDa); eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa; PPP1R67; protein phosphatase 1; protein phosphatase 1, regulatory subunit 67; regulatory subunit 67
2810026E11Rik; 38kDa; AA408636; AA571381; AA986487; AW822225; D2Ertd303e; eIF-2-beta; EIF2; EIF2B; EIF2beta; EIF2S2; PPP1R67