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Immunofluorescent staining of HepG2 cells using PA5-19557, anti-EIF2AK1 antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 5 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human EIF2AK1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with cynomolgus monkey based on sequence homology.
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
heme regulated initiation factor 2 alpha kinase; heme sensitive initiation factor 2a kinase; heme-controlled repressor; heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; heme-regulated inhibitor; heme-regulated initiation factor 2-alpha kinase; heme-regulated repressor; hemin-sensitive initiation factor 2-alpha kinase; PRO1362, HCR, HRI, KIAA1369, heme regulated initiation factor 2 alpha kinase
EIF2AK1; HCR; HRI; KIAA1369; PRO1362