Immunofluorescent analysis of EIF2B delta showing staining in the cytoplasm of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an EIF2B delta polyclonal antibody (Product # PA5-30292) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 187 of Human EIF2B delta|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30292 targets EIF2B delta in WB applications and shows reactivity with Human samples.
The PA5-30292 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 187 of Human EIF2B delta.
Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.
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