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Invitrogen
EIF4A1/EIF4A2/EIF4A3 Polyclonal Antibody
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Product Details
PA5-116950
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A (NP_0014071)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.638 mg/mL
Amount
163.8 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: 293T, HeLa, HepG2, MCF7, C2C12, Mouse brain, Rat brain
Immunogen sequence: MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA
Target Information
The eIF4A proteins are involved in many aspects of RNA metabolism due to their RNA-binding capacity and ATPase activity. eIF4A1 and eIF4A2 are cytoplasmic, whereas eIF4A3 is nuclear-localized. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-1; ATP-dependent RNA helicase eIF4A-2; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; eIF-4A-I; eIF-4A-II; eIF-4A-III; eIF4A-I; eIF4A-II; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4A-II; Eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A, isoform 1; eukaryotic translation initiation factor 4A, isoform 2; eukaryotic translation initiation factor 4A, isoform 3; initiation factor eIF-4A long form; KIAA0111; NMP 265; Nuclear matrix protein 265; protein synthesis initiation factor 4AII; unnamed protein product
Gene Aliases: 2400003O03Rik; 4833432N07Rik; BM-010; DDX2A; DDX2B; DDX48; EIF-4A; eIF-4A-I; eIF-4A-II; eIF-4A-III; Eif4; EIF4A; eIF4A-I; eIF4A-II; eIF4A-III; EIF4A1; EIF4A2; EIF4A3; eIF4AIII; EIF4F; Fal1; KIAA0111; mKIAA0111; MUK34; NEDHSS; NMP265; NUK34; RCPS
UniProt ID: (Human) P60842, (Human) Q14240, (Human) P38919, (Mouse) P60843, (Mouse) P10630, (Rat) Q5RKI1, (Mouse) Q91VC3, (Rat) Q3B8Q2
Entrez Gene ID: (Human) 1973, (Human) 1974, (Human) 9775, (Mouse) 13681, (Rat) 287436, (Mouse) 13682, (Rat) 303831, (Mouse) 192170, (Rat) 688288
nucleotide binding
RNA cap binding
nucleic acid binding
RNA binding
RNA helicase activity
double-stranded RNA binding
mRNA binding
translation initiation factor activity
helicase activity
protein binding
ATP binding
translation factor activity, RNA binding
hydrolase activity
ATPase activity
poly(A) binding
selenocysteine insertion sequence binding
RNA stem-loop binding
ribonucleoprotein complex binding
ATP-dependent RNA helicase activity
poly(A) RNA binding
molecular_function
cytoplasmic translational initiation
translation
translational initiation
positive regulation of transcription from RNA polymerase II promoter
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
mRNA splicing, via spliceosome
rRNA processing
mRNA processing
mRNA export from nucleus
regulation of translation
associative learning
RNA splicing
negative regulation of gene expression
negative regulation of translation
exploration behavior
positive regulation of translation
embryonic cranial skeleton morphogenesis
mRNA transport
cellular response to selenite ion
negative regulation of excitatory postsynaptic potential
regulation of translation at postsynapse, modulating synaptic transmission
negative regulation of selenocysteine incorporation
cellular response to brain-derived neurotrophic factor stimulus
regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
regulation of translational initiation
negative regulation of RNA-directed RNA polymerase activity
cellular response to leukemia inhibitory factor
regulation of gene expression
RNA secondary structure unwinding
organ regeneration
positive regulation of mRNA splicing, via spliceosome
regulation of alternative mRNA splicing, via spliceosome
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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