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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000-1:2,000 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
Flow Cytometry (Flow) |
1:100 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Dog, Human |
Host/Isotype |
Mouse / IgG2b |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OTI1B8 |
Immunogen |
Full length human recombinant protein of human EMG1 produced in HEK293T cell. |
Conjugate |
Unconjugated |
Form |
lyophilized |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 8% trehalose |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
EMG1 involved in 40S ribosomal subunit biogenesis. It seems to play a role a methylation reaction in pre-rRNA processing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 18S rRNA (pseudouridine(1248)-N1)-methyltransferase; 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1; 18S rRNA Psi1248 methyltransferase; EMG1 N1-specific pseudouridine methyltransferase; EMG1 nucleolar protein homolog; essential for mitotic growth 1; nucleolar essential protein 1; Nucleolar protein EMG1 homolog; Protein C2f; Ribosomal RNA small subunit methyltransferase NEP1; Ribosome biogenesis protein NEP1
Gene Aliases: C2F; EMG1; Grcc2f; NEP1
UniProt ID: (Human) Q92979
Entrez Gene ID: (Human) 10436, (Dog) 477708
Molecular Function:
methyltransferase
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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