|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Synthetic peptide derived from the C-terminal region of human Emi1 (early mitotic inhibitor 1, Fbx5) protein|
|Storage buffer||tissue culture supernatant|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
F-box proteins, critical components of the conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress.
37-6600 was used in western blot to study APC/C(Cdh1) function
|Ercilla A,Llopis A,Feu S,Aranda S,Ernfors P,Freire R,Agell N||Nucleic acids research (44:4745)||2016|
||Substrate degradation by the anaphase promoting complex occurs during mitotic slippage.||Lee J,Kim JA,Margolis RL,Fotedar R||Cell cycle (Georgetown, Tex.) (9:1792)||2010|
MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C.
37-6600 was used in western blot to study the effect of MASTL on cyclin B1 degradation and its mechanism
|Voets E,Wolthuis R||Biology open (4:484)||2015|
Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis.
37-6600 was used in western blot to study the effect of human papillomavirus type 16 E7 oncoprotein on the anaphase promoting complex/cyclosome and its mechanism
|Yu Y,Munger K||Virology (446:251)||2013|
The anaphase-promoting complex or cyclosome supports cell survival in response to endoplasmic reticulum stress.
37-6600 was used in western blot to investigate the regulation and function of APC/C(Cdh) in non-genotoxic stress responses.
|Chen M,Gutierrez GJ,Ronai ZA||PloS one (7:null)||2012|
Regulation of the action of early mitotic inhibitor 1 on the anaphase-promoting complex/cyclosome by cyclin-dependent kinases.
37-6600 was used in western blot to identify mechanisms other than degradation of Emi1, which release APC/C and promote entry into mitosis.
|Moshe Y,Bar-On O,Ganoth D,Hershko A||The Journal of biological chemistry (286:16647)||2011|
|Human||Not Cited||Substrate degradation by the anaphase promoting complex occurs during mitotic slippage.||Lee J,Kim JA,Margolis RL,Fotedar R||Cell cycle (Georgetown, Tex.) (9:1792)||2010|
|Not Applicable||Not Cited||
DNA damage triggers p21WAF1-dependent Emi1 down-regulation that maintains G2 arrest.
37-6600 was used in western blot to assess the role of p21(WAF1) in G2-M phase checkpoint control and in prevention of polyploidy after DNA damage
|Lee J,Kim JA,Barbier V,Fotedar A,Fotedar R||Molecular biology of the cell (20:1891)||2009|
|Human||Not Cited||Selective enhancing effect of early mitotic inhibitor 1 (Emi1) depletion on the sensitivity of doxorubicin or X-ray treatment in human cancer cells.||Shimizu N,Nakajima NI,Tsunematsu T,Ogawa I,Kawai H,Hirayama R,Fujimori A,Yamada A,Okayasu R,Ishimaru N,Takata T,Kudo Y||The Journal of biological chemistry (288:17238)||2013|