Western blot analysis of Non Neuronal Enolase using anti-Non Neuronal Enolase monoclonal antibody (MA5-17262) on (Lane 1) HeLa Cell lysate, (Lane 2) SK-N-MC Cell lysate, (Lane 3) K562 Cell lysate, (Lane 4) A431 Cell lysate, (Lane 5) HepG2 Cell lysate and (Lane 6) U2OS Cell lysate.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant human protein purified from E.coli (His-NNE)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 ug|
|Western Blot (WB)||0.01 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is A431 cell lysate.
Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.