Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.
Applications Reported: This Dan11mag antibody has been reported for use in immunohistochemistry of formalin-fixed paraffin embedded tissue sections, intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated Dan11mag is recommended for use in intracellular flow cytometry.).
Applications Tested: This Dan11mag antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse splenocytes and/or western blotting at 5 µg/mL. This Dan11mag antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low pH antigen retrieval at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2
Gene Aliases: C77258; Eomes; TBR-2; Tbr2
UniProt ID: (Mouse) O54839
Entrez Gene ID: (Mouse) 13813
Molecular Function: Rel homology transcription factor