Invitrogen

EOMES Monoclonal Antibody (Dan11mag), Alexa Fluor™ 488, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

40 Published Figures

42 References

View all (31) EOMES antibodies

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Protocols

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EOMES Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 eFluor® 450 (Product # 48-5941-82) followed by intracellular staining with 0.25 µg of Rat IgG2a K Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (left) or 0.25 µg of Anti-Mouse EOMES Alexa Fluor® 488 (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.

EOMES Antibody in Immunocytochemistry (ICC/IF) — 10.1371/journal.pgen.1009334.g002 Fig 2 Cep55 regulates cell fate of radial glial and intermediate progenitor cells, and neurons. (A) Representative images of radial glial cells (RGC; Pax6+) at VZ, intermediate progenitor cells (IPC; Tbr2+) at SVZ and total cells (DAPI) at E14.5, scale = 15 mum (upper panel). Quantification of percentage of RGCs (Pax6+, p< 0.0016) and IPCs (Tbr2+, p< 0.0337) in Cep55 +/+ and Cep55 -/- neocortices (Ncx) at E14.5 (lower). (B) Representative images of IPCs (Tbr2+) at SVZ, post-mitotic neurons (Tbr1+) at CP and total cells (DAPI, gray) at E14.5, scale = 15 mum (upper), quantification of the percentage of post-mitotic-neurons (Tbr1+, p< 0.0005) in Cep55 +/+ and Cep55 -/- neocortices at E14.5 (lower). (C) Neuron-specific class III beta-tubulin (Tuj1, p< 0.0037), relative intensity of Tuj1 staining quantified within a 100 mum 2 field of view. (D) Phosphohistone H3 (pH3; mitotic cells) in the VZ and SVZ, co-stained with Tbr2 to identify proliferating IPCs at E14.5, scale = 15 mum. Quantification of the percentage of total cells expressing pH3 to show the mitotic index in Vz, p< 0.0090; and SVZ, P< 0.0070. (E) Proliferating cells (Ki67+), IPCs (Tbr2+, green) delineating the SVZ, and total cells (DAPI) at E14.5, scale = 15 mum (upper), Comparison of the proportion of Ki67+ cells in Cep55 +/+ and Cep55 -/- neocortices to show proliferation index (lower, p< 0.0034). (F) Apoptotic cells (TUNEL) and total cells (DAPI) in the Ncx, at E14.5, scale = 15 mum (

EOMES Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Fig. 1 Altered aRG and IP population size in the Mir146 -/- brains. a Sagittal sections from Mir146a +/+ and Mir146a -/- brains at E14.5 labeled with PAX6, TBR2, and merge. b Boxplot showing the number of PAX6 + cells (+-S.D.) counted using 3 images from comparable plane per mouse. c Boxplot showing the quantification of TBR2 + cells (+-S.D.) from the same images as b . d Boxplot showing the ratio of TBR2 + / PAX6 + cells. e Phospho-histone 3 (PH3) stains dividing cells in the neocortex at E14.5 (white arrows). f Graph showing the average number of PH3 + cells (+-S.D.) in the SVZ. * P < 0.05 by Student's unpaired 2-tailed t test. g BrdU staining in the neocortex at E14.5 after 2h pulse chase; images were divided into five equal bins for counting. h Percentage of BrdU+ cells (+-S.D.) per bin (normalized against the total number of labeled cells). NS not significant; * p <= 0.05, ** p <= 0.01, *** p <= 0.001 by Mann-Whitney U test

EOMES Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Fig. 7. Lack of RA impairs cell migration of early - born cortical neurons. Brain sections from E17.5 mice electroporated at E13.5 with a GFP reporter construct were analysed for GFP (C-D',G-H'), and by immunolabelling for Tbr2 and Ctip2 (A-B',E-F'). Single-color immunolabelling for the two markers help to define the upper layers (UL) and deeper layers (DL) of the cortical plate and the intermediate zone (IZ). (I,J) Histograms depict the percentage of GFP-positive cells per zone (UL, DL and IZ) of the rostral (I) and caudal (J) levels of developing cortex. (K-L',O) Quantification of GFP-positive cells (green) expressing Tbr1 (red) in the cortical plate of control and Raldh2cKO animals. (M-N',P) Quantification of GFP-positive cells (green) expressing Ctip2 (red) in the cortical plate of control and Raldh2cKO animals. The histograms (O,P) show the percentage of double-labelled cells over GFP+ cells. Data presented as mean+-s.e.m. ; n =5 brains; * P <0.05, ** P <0.01, *** P <0.001 by two-way ANOVA (I,J) and two-tailed Student's t -test (O,P). Scale bars: 100 mum.

EOMES Antibody in Immunohistochemistry (IHC) — Figure 2. Baf53a deletion impairs the maintenance of neural stem and progenitor populations in the germinal zones of the cortex. ( A , B , left ) Schematic of mouse cortical neurogenesis depicting cortical radial glia (brown), and their progeny, newly generated (PAX6+TBR2+) and differentiating (PAX6-TBR2+) intermediate progenitor cells (tan) and migratory neuroblasts (orange), as well as cell type-specific markers expressed by each population. Immunostaining for the proliferation marker KI67 ( red ; A ), or the M-phase marker phospho-histone H3 (PH3; red; B ), showing colocalization with the cell apoptosis marker cleaved caspase 3 (CC3, green) in E15.5 BAF53a mutant cortex. Scale bar, 100 um. ( C ) Colabeling for the radial glia and intermediate progenitor markers PAX6 (green) and TBR2 (red), respectively, depicts disorganization of the stem and progenitor pools in the ventricular and subventricular zones (VZ/SVZ; insets ) in E15.5 BAF53a mutant brains. White arrowheads point to TBR2+ cells, highlighting the presence of IPCs in the VZ of mutant brains, and blue arrowheads point to PAX6+ cells further away from the ventricle in mutant mice compared with controls. Scale bar, 100 um. ( D ) Immunostaining for the radial glia and intermediate progenitor markers phospho-Vimentin (pVIM, cyan) and TBR2 (amber or white), respectively, further highlights the disorganization of the VZ/SVZ in E15.5 BAF53a mutant cortex, as well as the presence of many cells in the SVZ coexpressing both m

EOMES Antibody — Figure 2 IL-12 can enhance CD8 + Tem cells differentiation through T-bet and Eomes influenced by mTOR activated.The peripheral blood mononuclear cells (PBMC) obtained from IPA model mice, IPA treated with IL-12 or rapamycin and control animals were detected using flow cytometry after 7 days Aspergillus fumigatus inhaled by the nose. The side scatter (SSC) and CD8a were applied to gate CD8 + T lymphocytes, CD44 + CD45 + CD62 +-/- represented effective memory T cell (Tem). And then the TEMs were stained with IFN-gamma, mTOR, S6K, T-BET, EOMES. The data were presented as mean +- S.D. *: p < 0.05; **: p < 0.01; ***: p < 0.001. {TM}

Product Details

53-4875-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.5 µg/test	View 17 publications 17 publications
Immunocytochemistry (ICC/IF)	-	View 9 publications 9 publications
Immunohistochemistry (IHC)	-	View 14 publications 14 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	Dan11mag
Conjugate	Alexa Fluor™ 488 Alexa Fluor 660 APC eFluor 450 eFluor 660 PE PE-Cyanine5 PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Unconjugated
Excitation/Emission Max	499/520 nm View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10854265

Product Specific Information

Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This Dan11mag antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This Dan11mag antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5521) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

CD8⁺ T cells specific for cryptic apoptosis-associated epitopes exacerbate experimental autoimmune encephalomyelitis.

Cell death & disease

Feizi N,Focaccetti C,Pacella I,Tucci G,Rossi A,Costanza M,Pedotti R,Sidney J,Sette A,La Rocca C,Procaccini C,Matarese G,Barnaba V,Piconese S

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Cep55 regulation of PI3K/Akt signaling is required for neocortical development and ciliogenesis.

PLoS genetics

Rashidieh B,Shohayeb B,Bain AL,Fortuna PRJ,Sinha D,Burgess A,Mills R,Adams RC,Lopez JA,Blumbergs P,Finnie J,Kalimutho M,Piper M,Hudson JE,Ng DCH,Khanna KK

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunocytochemistry

Fri Oct 01 00:00:00 EDT 2021

Coordination of asparagine uptake and asparagine synthetase expression modulates CD8+ T cell activation.

JCI insight

Hope HC,Brownlie RJ,Fife CM,Steele L,Lorger M,Salmond RJ

53-4875-82 was used in Flow Cytometry to define the requirements for uptake of extracellular asparagine and the activity of the glutamine-hydrolyzing enzyme asparagine synthetase in CD8+ T cell activation.

Mon May 10 00:00:00 EDT 2021

Stage-dependent effects of intermittent hypoxia influence the outcome of hippocampal adult neurogenesis.

Scientific reports

Khuu MA,Nallamothu T,Castro-Rivera CI,Arias-Cavieres A,Szujewski CC,Garcia Iii AJ

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunocytochemistry

Tue Mar 16 00:00:00 EDT 2021

BAF subunit switching regulates chromatin accessibility to control cell cycle exit in the developing mammalian cortex.

Genes & development

Braun SMG,Petrova R,Tang J,Krokhotin A,Miller EL,Tang Y,Panagiotakos G,Crabtree GR

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunohistochemistry

Mon Mar 01 00:00:00 EST 2021

LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.

Nature communications

Hu HB,Song ZQ,Song GP,Li S,Tu HQ,Wu M,Zhang YC,Yuan JF,Li TT,Li PY,Xu YL,Shen XL,Han QY,Li AL,Zhou T,Chun J,Zhang XM,Li HY

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunohistochemistry

Thu Jan 28 00:00:00 EST 2021

Developmental exposure to diesel exhaust upregulates transcription factor expression, decreases hippocampal neurogenesis, and alters cortical lamina organization: relevance to neurodevelopmental disorders.

Journal of neurodevelopmental disorders

Cole TB,Chang YC,Dao K,Daza R,Hevner R,Costa LG

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunohistochemistry

Wed Dec 16 00:00:00 EST 2020

A Fas-4-1BB fusion protein converts a death to a pro-survival signal and enhances T cell therapy.

The Journal of experimental medicine

Oda SK,Anderson KG,Ravikumar P,Bonson P,Garcia NM,Jenkins CM,Zhuang S,Daman AW,Chiu EY,Bates BM,Greenberg PD

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

The Spindle-Associated Microcephaly Protein, WDR62, Is Required for Neurogenesis and Development of the Hippocampus.

Frontiers in cell and developmental biology

Shohayeb B,Ho UY,Hassan H,Piper M,Ng DCH

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunohistochemistry

Tue Oct 13 00:00:00 EDT 2020

CPEB4-Dependent Neonate-Born Granule Cells Are Required for Olfactory Discrimination.

Frontiers in behavioral neuroscience

Tseng CS,Chou SJ,Huang YS

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunohistochemistry

Wed Sep 30 00:00:00 EDT 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Flow Cytometry

Tue Sep 22 00:00:00 EDT 2020

Reelin Mediates Hippocampal Cajal-Retzius Cell Positioning and Infrapyramidal Blade Morphogenesis.

Journal of developmental biology

Ha S,Tripathi PP,Daza RA,Hevner RF,Beier DR

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunohistochemistry

Fri Sep 18 00:00:00 EDT 2020

Inhibition of MICA and MICB Shedding Elicits NK-Cell-Mediated Immunity against Tumors Resistant to Cytotoxic T Cells.

Cancer immunology research

Ferrari de Andrade L,Kumar S,Luoma AM,Ito Y,Alves da Silva PH,Pan D,Pyrdol JW,Yoon CH,Wucherpfennig KW

53-4875 was used in Flow cytometry/Cell sorting to investigate if resistant tumours can be targeted by natural killer cells with sufficient activating signals.

Mon Jun 01 00:00:00 EDT 2020

Intermediate progenitors support migration of neural stem cells into dentate gyrus outer neurogenic niches.

eLife

Nelson BR,Hodge RD,Daza RA,Tripathi PP,Arnold SJ,Millen KJ,Hevner RF

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunohistochemistry

Fri Apr 03 00:00:00 EDT 2020

Centrosome anchoring regulates progenitor properties and cortical formation.

Nature

Shao W,Yang J,He M,Yu XY,Lee CH,Yang Z,Joyner AL,Anderson KV,Zhang J,Tsou MB,Shi H,Shi SH

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunocytochemistry

Wed Apr 01 00:00:00 EDT 2020

Loss of the neurodevelopmental disease-associated gene miR-146a impairs neural progenitor differentiation and causes learning and memory deficits.

Molecular autism

Fregeac J,Moriceau S,Poli A,Nguyen LS,Oury F,Colleaux L

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunocytochemistry

Mon Mar 30 00:00:00 EDT 2020

Loss of the neurodevelopmental disease-associated gene miR-146a impairs neural progenitor differentiation and causes learning and memory deficits.

Molecular autism

Fregeac J,Moriceau S,Poli A,Nguyen LS,Oury F,Colleaux L

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunocytochemistry

Mon Mar 30 00:00:00 EDT 2020

The association of microcephaly protein WDR62 with CPAP/IFT88 is required for cilia formation and neocortical development.

Human molecular genetics

Shohayeb B,Ho U,Yeap YY,Parton RG,Millard SS,Xu Z,Piper M,Ng DCH

53-4875-80 was used in Immunocytochemistry to highlight the important role of the primary cilium in neocortical expansion and implicate ciliary dysfunction as underlying the pathology of MCPH2 patients.

Wed Jan 15 00:00:00 EST 2020

Aberrant calcium channel splicing drives defects in cortical differentiation in Timothy syndrome.

eLife

Panagiotakos G,Haveles C,Arjun A,Petrova R,Rana A,Portmann T,Paşca SP,Palmer TD,Dolmetsch RE

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunofluorescence

Mon Dec 23 00:00:00 EST 2019

Phenotype of NK-Like CD8(+) T Cells with Innate Features in Humans and Their Relevance in Cancer Diseases.

Frontiers in immunology

Barbarin A,Cayssials E,Jacomet F,Nunez NG,Basbous S,Lefèvre L,Abdallah M,Piccirilli N,Morin B,Lavoue V,Catros V,Piaggio E,Herbelin A,Gombert JM

Wed Nov 20 00:00:00 EST 2019

Lineage does not regulate the sensory synaptic input of projection neurons in the mouse olfactory bulb.

eLife

Sánchez-Guardado L,Lois C

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunofluorescence

Tue Aug 27 00:00:00 EDT 2019

EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming.

Nature communications

Istaces N,Splittgerber M,Lima Silva V,Nguyen M,Thomas S,Le A,Achouri Y,Calonne E,Defrance M,Fuks F,Goriely S,Azouz A

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Flow Cytometry

Wed Jul 24 00:00:00 EDT 2019

PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice.

eLife

Roy A,Murphy RM,Deng M,MacDonald JW,Bammler TK,Aldinger KA,Glass IA,Millen KJ

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunofluorescence

Thu May 16 00:00:00 EDT 2019

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Flow Cytometry

Tue Oct 30 00:00:00 EDT 2018

Interleukin-21 Induces Short-Lived Effector CD8⁺ T Cells but Does Not Inhibit Their Exhaustion after Mycobacterium bovis BCG Infection in Mice.

Infection and immunity

Noguchi N,Nakamura R,Hatano S,Yamada H,Sun X,Ohara N,Yoshikai Y

Wed Aug 01 00:00:00 EDT 2018

IL-12 Influence mTOR to Modulate CD8⁺ T Cells Differentiation through T-bet and Eomesodermin in Response to Invasive Pulmonary Aspergillosis.

International journal of medical sciences

Wang H,Li J,Han Q,Yang F,Xiao Y,Xiao M,Xu Y,Su L,Cui N,Liu D

Mon May 21 00:00:00 EDT 2018

IL-12 Influence mTOR to Modulate CD8⁺ T Cells Differentiation through T-bet and Eomesodermin in Response to Invasive Pulmonary Aspergillosis.

International journal of medical sciences

Wang H,Li J,Han Q,Yang F,Xiao Y,Xiao M,Xu Y,Su L,Cui N,Liu D

Mon May 21 00:00:00 EDT 2018

mTOR Modulates CD8+ T Cell Differentiation in Mice with Invasive Pulmonary Aspergillosis.

Open life sciences

Wang H,Xiao Y,Su L,Cui N,Liu D

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Guidelines for the use of flow cytometry and cell sorting in immunological studies.

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Akdis M,Andrä I,Annunziato F,Bacher P,Barnaba V,Battistini L,Bauer WM,Baumgart S,Becher B,Beisker W,Berek C,Blanco A,Borsellino G,Boulais PE,Brinkman RR,Büscher M,Busch DH,Bushnell TP,Cao X,Cavani A,Chattopadhyay PK,Cheng Q,Chow S,Clerici M,Cooke A,Cosma A,Cosmi L,Cumano A,Dang VD,Davies D,De Biasi S,Del Zotto G,Della Bella S,Dellabona P,Deniz G,Dessing M,Diefenbach A,Di Santo J,Dieli F,Dolf A,Donnenberg VS,Dörner T,Ehrhardt GRA,Endl E,Engel P,Engelhardt B,Esser C,Everts B,Dreher A,Falk CS,Fehniger TA,Filby A,Fillatreau S,Follo M,Förster I,Foster J,Foulds GA,Frenette PS,Galbraith D,Garbi N,García-Godoy MD,Geginat J,Ghoreschi K,Gibellini L,Goettlinger C,Goodyear CS,Gori A,Grogan J,Gross M,Grützkau A,Grummitt D,Hahn J,Hammer Q,Hauser AE,Haviland DL,Hedley D,Herrera G,Herrmann M,Hiepe F,Holland T,Hombrink P,Houston JP,Hoyer BF,Huang B,Hunter CA,Iannone A,Jäck HM,Jávega B,Jonjic S,Juelke K,Jung S,Kaiser T,Kalina T,Keller B,Khan S,Kienhöfer D,Kroneis T,Kunkel D,Kurts C,Kvistborg P,Lannigan J,Lantz O,Larbi A,LeibundGut-Landmann S,Leipold MD,Levings MK,Litwin V,Liu Y,Lohoff M,Lombardi G,Lopez L,Lovett-Racke A,Lubberts E,Ludewig B,Lugli E,Maecker HT,Martrus G,Matarese G,Maueröder C,McGrath M,McInnes I,Mei HE,Melchers F,Melzer S,Mielenz D,Mills K,Mirrer D,Mjösberg J,Moore J,Moran B,Moretta A,Moretta L,Mosmann TR,Müller S,Müller W,Münz C,Multhoff G,Munoz LE,Murphy KM,Nakayama T,Nasi M,Neudörfl C,Nolan J,Nourshargh S,O'Connor JE,Ouyang W,Oxenius A,Palankar R,Panse I,Peterson P,Peth C,Petriz J,Philips D,Pickl W,Piconese S,Pinti M,Pockley AG,Podolska MJ,Pucillo C,Quataert SA,Radstake TRDJ,Rajwa B,Rebhahn JA,Recktenwald D,Remmerswaal EBM,Rezvani K,Rico LG,Robinson JP,Romagnani C,Rubartelli A,Ruckert B,Ruland J,Sakaguchi S,Sala-de-Oyanguren F,Samstag Y,Sanderson S,Sawitzki B,Scheffold A,Schiemann M,Schildberg F,Schimisky E,Schmid SA,Schmitt S,Schober K,Schüler T,Schulz AR,Schumacher T,Scotta C,Shankey TV,Shemer A,Simon AK,Spidlen J,Stall AM,Stark R,Stehle C,Stein M,Steinmetz T,Stockinger H,Takahama Y,Tarnok A,Tian Z,Toldi G,Tornack J,Traggiai E,Trotter J,Ulrich H,van der Braber M,van Lier RAW,Veldhoen M,Vento-Asturias S,Vieira P,Voehringer D,Volk HD,von Volkmann K,Waisman A,Walker R,Ward MD,Warnatz K,Warth S,Watson JV,Watzl C,Wegener L,Wiedemann A,Wienands J,Willimsky G,Wing J,Wurst P,Yu L,Yue A,Zhang Q,Zhao Y,Ziegler S,Zimmermann J

53-4875 was used in Flow cytometry/Cell sorting to collect the currently accepted best methods for monitoring most of the variation of the major players of immune system.

Sun Oct 01 00:00:00 EDT 2017

A dual-fluorescence reporter in the Eomes locus for live imaging and medium-term lineage tracing.

Genesis (New York, N.Y. : 2000)

Probst S,Daza RA,Bader N,Hummel JF,Weiß M,Tanriver Y,Hevner RF,Arnold SJ

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunocytochemistry

Tue Aug 01 00:00:00 EDT 2017

A dual-fluorescence reporter in the Eomes locus for live imaging and medium-term lineage tracing.

Genesis (New York, N.Y. : 2000)

Probst S,Daza RA,Bader N,Hummel JF,Weiß M,Tanriver Y,Hevner RF,Arnold SJ

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunocytochemistry

Tue Aug 01 00:00:00 EDT 2017

Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development.

Biology open

Haushalter C,Schuhbaur B,Dollé P,Rhinn M

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunohistochemistry (Frozen)

Wed Feb 15 00:00:00 EST 2017

Protein Translation Activity: A New Measure of Host Immune Cell Activation.

Journal of immunology (Baltimore, Md. : 1950)

Seedhom MO,Hickman HD,Wei J,David A,Yewdell JW

53-4875 was used in Flow cytometry/Cell sorting to describe the in vivo ribopuromycylation method.

Mon Aug 15 00:00:00 EDT 2016

Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Fame RM,MacDonald JL,Dunwoodie SL,Takahashi E,Macklis JD

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunocytochemistry

Wed Jun 15 00:00:00 EDT 2016

The G protein-coupled receptor GPR157 regulates neuronal differentiation of radial glial progenitors through the Gq-IP3 pathway.

Scientific reports

Takeo Y,Kurabayashi N,Nguyen MD,Sanada K

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunofluorescence

Wed May 04 00:00:00 EDT 2016

The Residual Innate Lymphoid Cells in NFIL3-Deficient Mice Support Suboptimal Maternal Adaptations to Pregnancy.

Frontiers in immunology

Boulenouar S,Doisne JM,Sferruzzi-Perri A,Gaynor LM,Kieckbusch J,Balmas E,Yung HW,Javadzadeh S,Volmer L,Hawkes DA,Phillips K,Brady HJ,Fowden AL,Burton GJ,Moffett A,Colucci F

53-4875-82 was used in immunohistochemistry (frozen) immunofluorescence to show that maternal NFIL3 mediates non-redundant functions in mouse reproduction.

Mon Feb 29 00:00:00 EST 2016

Active medulloblastoma enhancers reveal subgroup-specific cellular origins.

Nature

Lin CY,Erkek S,Tong Y,Yin L,Federation AJ,Zapatka M,Haldipur P,Kawauchi D,Risch T,Warnatz HJ,Worst BC,Ju B,Orr BA,Zeid R,Polaski DR,Segura-Wang M,Waszak SM,Jones DT,Kool M,Hovestadt V,Buchhalter I,Sieber L,Johann P,Chavez L,Gröschel S,Ryzhova M,Korshunov A,Chen W,Chizhikov VV,Millen KJ,Amstislavskiy V,Lehrach H,Yaspo ML,Eils R,Lichter P,Korbel JO,Pfister SM,Bradner JE,Northcott PA

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunofluorescence

Thu Feb 04 00:00:00 EST 2016

Activated NKT cells imprint NK-cell differentiation, functionality and education.

European journal of immunology

Riese P,Trittel S,May T,Cicin-Sain L,Chambers BJ,Guzmán CA

53-4875 was used in Flow cytometry/Cell sorting to study the cellular cross-talk between natural killer T cells and natural killer cells.

Mon Jun 01 00:00:00 EDT 2015

NK cell maturation and function in C57BL/6 mice are altered by caloric restriction.

Journal of immunology (Baltimore, Md. : 1950)

Clinthorne JF,Beli E,Duriancik DM,Gardner EM

53-4875 was used in Flow cytometry/Cell sorting to analyse of the effects of caloric intake on NK cell phenotype and function.

Tue Jan 15 00:00:00 EST 2013

Tbr2 deficiency in mitral and tufted cells disrupts excitatory-inhibitory balance of neural circuitry in the mouse olfactory bulb.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mizuguchi R,Naritsuka H,Mori K,Mao CA,Klein WH,Yoshihara Y

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunofluorescence

Wed Jun 27 00:00:00 EDT 2012

Tbr2 deficiency in mitral and tufted cells disrupts excitatory-inhibitory balance of neural circuitry in the mouse olfactory bulb.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mizuguchi R,Naritsuka H,Mori K,Mao CA,Klein WH,Yoshihara Y

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Immunofluorescence

Wed Jun 27 00:00:00 EDT 2012

CD134 plus CD137 dual costimulation induces Eomesodermin in CD4 T cells to program cytotoxic Th1 differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Qui HZ,Hagymasi AT,Bandyopadhyay S,St Rose MC,Ramanarasimhaiah R,Ménoret A,Mittler RS,Gordon SM,Reiner SL,Vella AT,Adler AJ

Published figure using EOMES monoclonal antibody (Product # 53-4875-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2011

IFN-alpha beta and self-MHC divert CD8 T cells into a distinct differentiation pathway characterized by rapid acquisition of effector functions.

Journal of immunology (Baltimore, Md. : 1950)

Marshall HD,Prince AL,Berg LJ,Welsh RM

Sun Aug 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2

Gene Aliases: C77258; Eomes; TBR-2; Tbr2

UniProt ID: (Mouse) O54839

Entrez Gene ID: (Mouse) 13813

Molecular Function: Rel homology transcription factor

Function(s)

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification blastocyst development trophectodermal cell differentiation CD8-positive, alpha-beta T cell differentiation involved in immune response immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development gastrulation brain development cardioblast differentiation regulation of gene expression stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation cell differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding

EOMES Monoclonal Antibody (Dan11mag), Alexa Fluor™ 488, eBioscience™

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53-4875-82

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Immunocytochemistry (ICC/IF)

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Immunohistochemistry (Frozen) (IHC (F))

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