Invitrogen

EOMES Monoclonal Antibody (Dan11mag), PE-eFluor™ 610, eBioscience™

Product Image Gallery

EOMES Antibody in Flow Cytometry (Flow) — Surface staining of C57Bl/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82) followed by intracellularly staining with 0.25 µg of Rat IgG2a K Isotype Control PE-eFluor® 610 (Product # 61-4321-82) (left) or 0.25 µg of Anti-Mouse EOMES PE-eFluor® 610 (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.

Product Details

61-4875-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.5 µg/test	View 15 publications 15 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	Dan11mag
Conjugate	PE-eFluor™ 610 Alexa Fluor 488 Alexa Fluor 660 APC eFluor 450 eFluor 660 PE PE-Cyanine5 PE-Cyanine7 PerCP-eFluor 710 Unconjugated
Excitation/Emission Max	565/606 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2574614

Product Specific Information

Description: This Dan11mag antibody recognizes Eomesodermin (EOMES), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This Dan11mag antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Dan11mag antibody has been tested by intracellular staining and flow cytometric of mouse splenocytes analysis using Foxp3/Transcription Factor Buffer Staining Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Interleukin-1β secretion induced by mucosa-associated gut commensal bacteria promotes intestinal barrier repair.

Gut microbes

Wu WH,Kim M,Chang LC,Assie A,Saldana-Morales FB,Zegarra-Ruiz DF,Norwood K,Samuel BS,Diehl GE

61-4875-82 was used in Flow cytometry/Cell sorting to demonstrate an unexpected role for commensal bacteria in promoting IL-1β secretion to support intestinal barrier repair.

Thu Feb 24 00:00:00 EST 2022

The transcription factor HIF-1α mediates plasticity of NKp46+ innate lymphoid cells in the gut.

The Journal of experimental medicine

Krzywinska E,Sobecki M,Nagarajan S,Zacharjasz J,Tambuwala MM,Pelletier A,Cummins E,Gotthardt D,Fandrey J,Kerdiles YM,Peyssonnaux C,Taylor CT,Sexl V,Stockmann C

61-4875-82 was used in Flow cytometry/Cell sorting to suggest that HIF-1α shapes the ILC phenotype in the gut.

Mon Feb 07 00:00:00 EST 2022

CD8⁺ T cells specific for cryptic apoptosis-associated epitopes exacerbate experimental autoimmune encephalomyelitis.

Cell death & disease

Feizi N,Focaccetti C,Pacella I,Tucci G,Rossi A,Costanza M,Pedotti R,Sidney J,Sette A,La Rocca C,Procaccini C,Matarese G,Barnaba V,Piconese S

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 ^iCreTbx21 ^fl/fl mice.

Wellcome open research

Cuff AO,Male V

61-4875 was used in Flow cytometry/Cell sorting to examine the role of Tbet-dependent type I innate lymphoid cells in the murine liver.

Sat Jan 09 00:00:00 EST 2021

A Fas-4-1BB fusion protein converts a death to a pro-survival signal and enhances T cell therapy.

The Journal of experimental medicine

Oda SK,Anderson KG,Ravikumar P,Bonson P,Garcia NM,Jenkins CM,Zhuang S,Daman AW,Chiu EY,Bates BM,Greenberg PD

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

Hematopoietic Progenitor Kinase1 (HPK1) Mediates T Cell Dysfunction and Is a Druggable Target for T Cell-Based Immunotherapies.

Cancer cell

Si J,Shi X,Sun S,Zou B,Li Y,An D,Lin X,Gao Y,Long F,Pang B,Liu X,Liu T,Chi W,Chen L,Dimitrov DS,Sun Y,Du X,Yin W,Gao G,Min J,Wei L,Liao X

61-4875-82 was used in Flow cytometry/Cell sorting to investigate if the HPK1-NFκB-Blimp1 axis mediates T cell dysfunction.

Mon Oct 12 00:00:00 EDT 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

61-4875 was used in Flow cytometry/Cell sorting to provide further insights into vaccine-induced multifaceted mucosal T cell immunity with implications in the development of vaccines against respiratorypathogens, including influenza virus and SARS-CoV-2.

Tue Sep 22 00:00:00 EDT 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

Tue Sep 22 00:00:00 EDT 2020

Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

Huang A,Shinde PV,Huang J,Senff T,Xu HC,Margotta C,Häussinger D,Willnow TE,Zhang J,Pandyra AA,Timm J,Weggen S,Lang KS,Lang PA

61-4875 was used in Flow cytometry/Cell sorting to show that progranulin (PGRN) can reduce NK cell cytotoxicity through reduction of NK cell expansion, granzyme B transcription, and NK cell-mediated lysis of target cells.

Thu Sep 05 00:00:00 EDT 2019

EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming.

Nature communications

Istaces N,Splittgerber M,Lima Silva V,Nguyen M,Thomas S,Le A,Achouri Y,Calonne E,Defrance M,Fuks F,Goriely S,Azouz A

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Wed Jul 24 00:00:00 EDT 2019

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Tue Oct 30 00:00:00 EDT 2018

IL-12 Influence mTOR to Modulate CD8⁺ T Cells Differentiation through T-bet and Eomesodermin in Response to Invasive Pulmonary Aspergillosis.

International journal of medical sciences

Wang H,Li J,Han Q,Yang F,Xiao Y,Xiao M,Xu Y,Su L,Cui N,Liu D

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Mon May 21 00:00:00 EDT 2018

mTOR Modulates CD8+ T Cell Differentiation in Mice with Invasive Pulmonary Aspergillosis.

Open life sciences

Wang H,Xiao Y,Su L,Cui N,Liu D

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

CXCR6 marks a novel subset of T-bet(lo)Eomes(hi) natural killer cells residing in human liver.

Scientific reports

Stegmann KA,Robertson F,Hansi N,Gill U,Pallant C,Christophides T,Pallett LJ,Peppa D,Dunn C,Fusai G,Male V,Davidson BR,Kennedy P,Maini MK

61-4875-82 was used in Flow cytometry/Cell sorting to identify surface expression of CXCR6 as a marker of a novel subset of T-bet(lo)Eomes(hi) natural killer cells residing in the human liver.

Mon May 23 00:00:00 EDT 2016

CD134 plus CD137 dual costimulation induces Eomesodermin in CD4 T cells to program cytotoxic Th1 differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Qui HZ,Hagymasi AT,Bandyopadhyay S,St Rose MC,Ramanarasimhaiah R,Ménoret A,Mittler RS,Gordon SM,Reiner SL,Vella AT,Adler AJ

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2011

IFN-alpha beta and self-MHC divert CD8 T cells into a distinct differentiation pathway characterized by rapid acquisition of effector functions.

Journal of immunology (Baltimore, Md. : 1950)

Marshall HD,Prince AL,Berg LJ,Welsh RM

Sun Aug 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2

Gene Aliases: C77258; Eomes; TBR-2; Tbr2

UniProt ID: (Mouse) O54839

Entrez Gene ID: (Mouse) 13813

Molecular Function: Rel homology transcription factor

Function(s)

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification blastocyst development trophectodermal cell differentiation CD8-positive, alpha-beta T cell differentiation involved in immune response immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development gastrulation brain development cardioblast differentiation regulation of gene expression stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation cell differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding

EOMES Monoclonal Antibody (Dan11mag), PE-eFluor™ 610, eBioscience™

Product Details

61-4875-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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