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Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1-5 µg/mL | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
3 µg/mL | - |
ELISA (ELISA) |
10 µg/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
1B1 |
Immunogen |
EP300 (NP_001420, 731 a.a. approximately 830 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
See Label |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.4 |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Sequence of this protein is as follows: PPLQHHGQLA QPGALNPPMG YGPRMQQPSN QGQFLPQTQF PSQGMNVTNI PLAPSSGQAP VSQAQMSSSS CPVNSPIMPP GSQGSHIHCP QLPQPALHQN
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E1A-associated protein p300; E1A-binding protein, 300kD; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; p300 HAT; Protein 2-hydroxyisobutyryltransferase p300; Protein lactyltransferas p300; Protein propionyltransferase p300
Gene Aliases: EP300; KAT3B; P300; RSTS2
UniProt ID: (Human) Q09472
Entrez Gene ID: (Human) 2033
Molecular Function:
histone modifying enzyme
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