Invitrogen

EPHX2 Monoclonal Antibody (OTI1H5)

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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EPHX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a EPHX2 monoclonal antibody (Product # MA5-25223).

EPHX2 Antibody in Western Blot (WB) — Western blot was performed using Anti-EPHX2 Monoclonal Antibody (OTI1H5) (Product # MA5-25223) and a 60 kDa band corresponding to bifunctional Epoxide Hydrolase 2 (EPHX2) was observed across mouse tissues tested except skeletal muscle. Whole cell extracts (30 µg lysate) of Mouse Brown Fat (Lane 1), Mouse Adipose (Lane 2), Mouse Liver (Lane 3), Mouse Kidney (Lane 4), Mouse Heart (Lane 5) and Mouse Skeletal Muscle (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). A band corresponding to tissue IgG was observed around ~25 kDa.

EPHX2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of bifunctional epoxide hydrolase 2 (EPHX2) was observed in high expression cell lines K-562, Hep G2, LNCaP, DU 145 and PC-3 and not expressed in null cell lines U-87 MG, A-673 and HeLa using Anti-EPHX2 Monoclonal Antibody (OTI1H5) (Product # MA5-25223) in Western Blot. {RE}

Product Details

MA5-25223

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1000-1:2000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI1H5
Immunogen	Full length human recombinant protein of EPHX2 produced in HEK293T cell View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2723132

Target Information

This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bifunctional epoxide hydrolase 2; CEH; Cytosolic epoxide hydrolase 2; Epoxide hydratase; epoxide hydrolase 2, cytoplasmic; epoxide hydrolase 2, cytosolic; epoxide hydrolase, soluble; Lipid-phosphate phosphatase; SEH; Soluble epoxide hydrolase

Gene Aliases: CEH; EPHX2; SEH

UniProt ID: (Human) P34913

Entrez Gene ID: (Human) 2053

Molecular Function: hydrolase

Function(s)

xenobiotic metabolic process cellular calcium ion homeostasis inflammatory response regulation of blood pressure response to toxic substance positive regulation of gene expression dephosphorylation drug metabolic process epoxygenase P450 pathway cholesterol homeostasis positive regulation of vasodilation stilbene catabolic process phospholipid dephosphorylation reactive oxygen species metabolic process regulation of cholesterol metabolic process

Process(es)

magnesium ion binding epoxide hydrolase activity receptor binding toxic substance binding phosphatase activity 10-hydroxy-9-(phosphonooxy)octadecanoate phosphatase activity lipid phosphatase activity protein homodimerization activity

EPHX2 Monoclonal Antibody (OTI1H5)

Product Details

MA5-25223

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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