Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human heart stained using a EPM2A/Laforin polyclonal antibody (Product # PA5-23252) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Bovine, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (93 PGGELSWEGNGPHHDRC 109) of EPM2A/Laforin protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||0.25-0.50 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
EPM2A/Laforin is a novel dual-specificity phosphatase which associates with polyribosomes and belongs to the protein-tyrosine phosphatase family. Two isoforms of Laforin are reported which have alternate C termini and a common segment that consists of a carbohydrate-binding module and a dual-specificity protein phosphatase domain. Laforin plays an important role in the regulation of glycogen metabolism, prevents the formation of polyglucasans, and hydrolyze phosphotyrosine and phosphoserine/threonine substrates. Malin, a ubiquitin ligase, helps in regulation of Laforin protein concentration. Laforin not only interacts with itself but also with PPP1R5, HIRIP5 and EPM2AIP1. Mutations in the gene encoding EPM2A have been associated with myoclonic epilepsy type 2 (EPM2), also known as Lafora disease, an autosomal recessive and severe form of adolescent-onset progressive epilepsy. Multiple transcript variants of Laforin are expressed in most of the tissues, including brain.
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