Immunohistochemical analysis of EPO in immersion-fixed paraffin-embedded sections of human kidney cancer tissue using an EPO polyclonal antibody (Product # PA1-30182) at a dilution of 10 µg/mL overnight at 4°C (brown) and counterstained with hematoxylin (blue). Lower image shows samples without primary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length protein (Human), expressed in CHO cells.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30182 detects EPO from human samples.
PA1-30182 has been successfully used in indirect ELISA, IHC-P, and Western blot applications. PA1-30182 has been tested for its ability to neutralize the biological activity of EPO on the human cell line, TF1.
The PA1-30182 immunogen is recombinant full length protein (Human), expressed in CHO cells.
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.