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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Full length recombinant human ERCC1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13827 targets ERCC1 in IP and WB applications and shows reactivity with Human samples.
The MA5-13827 immunogen is full length recombinant human ERCC1 protein.
The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Divergent genomic and epigenomic landscapes of lung cancer subtypes underscore the selection of different oncogenic pathways during tumor development.
MA5-13827 was used in immunohistochemistry to study genomic and epigenomic differences between subtypes of non-small cell lung cancer and the clinical implications
|Lockwood WW,Wilson IM,Coe BP,Chari R,Pikor LA,Thu KL,Solis LM,Nunez MI,Behrens C,Yee J,English J,Murray N,Tsao MS,Minna JD,Gazdar AF,Wistuba II,MacAulay CE,Lam S,Lam WL||PloS one (7:null)||2012|
Development of an unsupervised pixel-based clustering algorithm for compartmentalization of immunohistochemical expression using Automated QUantitative Analysis.
MA5-13827 was used in immunohistochemistry to evaluate a novel image processing algorithm for immunohistochemical analysis
|Gustavson MD,Bourke-Martin B,Reilly DM,Cregger M,Williams C,Tedeschi G,Pinard R,Christiansen J||Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry (17:329)||2009|
Delayed c-Fos activation in human cells triggers XPF induction and an adaptive response to UVC-induced DNA damage and cytotoxicity.
MA5-13827 was used in western blot to study the role of delayed c-Fos activation in the adaptive response to UVC-induced DNA damage and cytotoxicity
|Tomicic MT,Reischmann P,Rasenberger B,Meise R,Kaina B,Christmann M||Cellular and molecular life sciences : CMLS (68:1785)||2011|
Subcellular distribution of a fluorescence-labeled combi-molecule designed to block epidermal growth factor receptor tyrosine kinase and damage DNA with a green fluorescent species.
MA5-13827 was used in western blot to study the subcellular distribution of a mixed EGFR-DNA targeting molecule utilizing a green labelled DNA-damaging moiety and a blue-labelled EGFR inhibitor
|Todorova MI,Larroque AL,Dauphin-Pierre S,Fang YQ,Jean-Claude BJ||Molecular cancer therapeutics (9:869)||2010|
The ERCC1/XPF endonuclease is required for completion of homologous recombination at DNA replication forks stalled by inter-strand cross-links.
MA5-13827 was used in western blot to investigate the function of ERCC1-XPF complex in inter-strand cross-link repair during DNA replication
|Al-Minawi AZ,Lee YF,Håkansson D,Johansson F,Lundin C,Saleh-Gohari N,Schultz N,Jenssen D,Bryant HE,Meuth M,Hinz JM,Helleday T||Nucleic acids research (37:6400)||2009|
ERCC1 expression and RAD51B activity correlate with cell cycle response to platinum drug treatment not DNA repair.
MA5-13827 was used in western blot to investigate the mechanism for platinum resistance in specific cell lines and the involvement of ERCC1 and RAD51B proteins
|Stordal B,Davey R||Cancer chemotherapy and pharmacology (63:661)||2009|
ZNRD1 might mediate UV irradiation related DNA damage and repair in human esophageal cancer cells by regulation of ERCC1.
MA5-13827 was used in western blot to study the potential role of ZNRD1 in mediating UV irradiation-related DNA damage and repair in human esophageal cancer cells
|Guo W,Zhao YP,Jiang YG,Wang RW,Hong L,Fan DM||Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus / I.S.D.E (21:730)||2008|
Enhanced cisplatin cytotoxicity by disturbing the nucleotide excision repair pathway in ovarian cancer cell lines.
MA5-13827 was used in western blot to study the increased cytotoxicity of cisplatin following antisense knockdown of the nucleotide excision repair pathway in ovarian cancer cell lines
|Selvakumaran M,Pisarcik DA,Bao R,Yeung AT,Hamilton TC||Cancer research (63:1311)||2003|
excision repair cross-complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
COFS4; ERCC1; RAD10; UV20