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Western blot analysis was performed on whole cell extracts (30 ug lysate) of HeLa (Lane 1), A431 (Lane 2) and KARPAS 299 (lane 3).The blots were probed with Anti-ERCC1 Mouse Monoclonal Antibody (Product# MA513830, 1-3 ug/ml) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 626520, 1:4000 dilution). A 36 kDa band corresponding to ERCC1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Full length recombinant human ERCC1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13830 targets ERCC1 in IP and WB applications and shows reactivity with Human samples.
The MA5-13830 immunogen is full length recombinant human ERCC1 protein.
The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Concept of Divergent Targeting through the Activation and Inhibition of Receptors as a Novel Chemotherapeutic Strategy: Signaling Responses to Strong DNA-Reactive Combinatorial Mimicries.
MA5-13830 was used in immunocytochemistry and western blot to study the concept of divergent targeting through the activation and inhibition of receptors as a novel chemotherapeutic strategy
|Watt HL,Rachid Z,Jean-Claude BJ||Journal of signal transduction (2012:null)||2012|
Delayed c-Fos activation in human cells triggers XPF induction and an adaptive response to UVC-induced DNA damage and cytotoxicity.
MA5-13830 was used in western blot to study the role of delayed c-Fos activation in the adaptive response to UVC-induced DNA damage and cytotoxicity
|Tomicic MT,Reischmann P,Rasenberger B,Meise R,Kaina B,Christmann M||Cellular and molecular life sciences : CMLS (68:1785)||2011|
Subcellular distribution of a fluorescence-labeled combi-molecule designed to block epidermal growth factor receptor tyrosine kinase and damage DNA with a green fluorescent species.
MA5-13830 was used in western blot to study the subcellular distribution of a mixed EGFR-DNA targeting molecule utilizing a green labelled DNA-damaging moiety and a blue-labelled EGFR inhibitor
|Todorova MI,Larroque AL,Dauphin-Pierre S,Fang YQ,Jean-Claude BJ||Molecular cancer therapeutics (9:869)||2010|
The ERCC1/XPF endonuclease is required for completion of homologous recombination at DNA replication forks stalled by inter-strand cross-links.
MA5-13830 was used in western blot to investigate the function of ERCC1-XPF complex in inter-strand cross-link repair during DNA replication
|Al-Minawi AZ,Lee YF,Håkansson D,Johansson F,Lundin C,Saleh-Gohari N,Schultz N,Jenssen D,Bryant HE,Meuth M,Hinz JM,Helleday T||Nucleic acids research (37:6400)||2009|
ERCC1 expression and RAD51B activity correlate with cell cycle response to platinum drug treatment not DNA repair.
MA5-13830 was used in western blot to investigate the mechanism for platinum resistance in specific cell lines and the involvement of ERCC1 and RAD51B proteins
|Stordal B,Davey R||Cancer chemotherapy and pharmacology (63:661)||2009|
ZNRD1 might mediate UV irradiation related DNA damage and repair in human esophageal cancer cells by regulation of ERCC1.
MA5-13830 was used in western blot to study the potential role of ZNRD1 in mediating UV irradiation-related DNA damage and repair in human esophageal cancer cells
|Guo W,Zhao YP,Jiang YG,Wang RW,Hong L,Fan DM||Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus / I.S.D.E (21:730)||2008|
Enhanced cisplatin cytotoxicity by disturbing the nucleotide excision repair pathway in ovarian cancer cell lines.
MA5-13830 was used in western blot to study the increased cytotoxicity of cisplatin following antisense knockdown of the nucleotide excision repair pathway in ovarian cancer cell lines
|Selvakumaran M,Pisarcik DA,Bao R,Yeung AT,Hamilton TC||Cancer research (63:1311)||2003|
Development of an unsupervised pixel-based clustering algorithm for compartmentalization of immunohistochemical expression using Automated QUantitative Analysis.
MA5-13830 was used in immunohistochemistry to evaluate a novel image processing algorithm for immunohistochemical analysis
|Gustavson MD,Bourke-Martin B,Reilly DM,Cregger M,Williams C,Tedeschi G,Pinard R,Christiansen J||Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry (17:329)||2009|
excision repair cross-complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
COFS4; ERCC1; RAD10; UV20