Invitrogen

ERCC1 Monoclonal Antibody (3H11)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

1 Published Figure

9 References

View all (81) ERCC1 antibodies

Datasheet

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Datasheet

Questions & Answers

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ERCC1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), A431 (Lane 2) and KARPAS 299 (lane 3).The blots were probed with Anti-ERCC1 Mouse Monoclonal Antibody (Product # MA5-13830, 1-3 µg/mL) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 36 kDa band corresponding to ERCC1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

ERCC1 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with ERCC1 siRNA and decrease in signal intensity was observed in western blot application using Anti-ERCC1 Mouse Monoclonal Antibody (Product# MA513830). {KD}

ERCC1 Antibody — Figure 5. TSP1 and ERCC1 are involved in Txr1-mediated L-OHP sensitivity. (A) Txr1, TSP1 and ERCC1 mRNA levels in SGC7901 cells following siCON or siTxr1 lentiviral transfection and L-OHP treatment, detected using qPCR (***P<0.001 vs. CON). (B) Txr1, TSP1 and ERCC1 protein levels, examined by western blotting and using beta-actin as a loading control. (C) Txr1, TSP1 and ERCC1 mRNA levels in SGC-7901 cells following control or Txr1 lentiviral transfection and L-OHP treatment, detected by qPCR (***P<0.001 vs. CON). (D) Txr1, TSP1 and ERCC1 protein levels, examined by western blotting and using beta-actin as a loading control. Txr-1, taxol resistance gene 1; CON, negative control; Lv, recombinant lentivirus; TSP1, thrombospondin-1; ERCC, excision repair cross-complementing 1 protein; L-OHP, oxaliplatin; si, small interfering; qPCR, quantitative polymerase chain reaction.

Product Details

Applications

Tested Dilution

Publications

Immunoprecipitation (IP)

2 µg/mL

Western Blot (WB)

1-3 µg/mL

View 7 publications 7 publications

Immunocytochemistry (ICC)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

3H11

Immunogen

Full length recombinant human ERCC1 protein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10982462

Product Specific Information

MA5-13830 targets ERCC1 in IP and WB applications and shows reactivity with Human samples.

The MA5-13830 immunogen is full length recombinant human ERCC1 protein.

Target Information

Anti-Human ERCC1 is an IgG2a Mouse Monoclonal Antibody that recognizes Excision Cross Complementing 1 (ERCC1), a human endonuclease. ERCC1 protein forms a complex with Xeroderma Pigmentosum Group F (XPF) protein. During nucleotide excision repair of damaged DNA, the ERCC1-XPF complex forms an incision at the 5' end of the DNA lesion. Deficiencies in the nucleotide excision repair pathway result in disorders such as Xeroderma pigmentosum, Cockayne's syndrome and trichothiodystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)

Gene Aliases: COFS4; ERCC1; RAD10; UV20

UniProt ID: (Human) P07992

Entrez Gene ID: (Human) 2067

Molecular Function: DNA binding protein endodeoxyribonuclease nuclease nucleic acid binding