Invitrogen

ERCC1 Monoclonal Antibody (3H11)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

9 References

View all (85) ERCC1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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ERCC1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), A431 (Lane 2) and KARPAS 299 (lane 3).The blots were probed with Anti-ERCC1 Mouse Monoclonal Antibody (Product # MA5-13830, 1-3 µg/mL) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 36 kDa band corresponding to ERCC1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

ERCC1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with ERCC1 siRNA and decrease in signal intensity was observed in western blot application using Anti-ERCC1 Mouse Monoclonal Antibody (Product# MA513830). {KD}

Product Details

MA5-13830

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	View 7 publications 7 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	2 µg/mL	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	3H11
Immunogen	Full length recombinant human ERCC1 protein View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10982462

Product Specific Information

MA5-13830 targets ERCC1 in IP and WB applications and shows reactivity with Human samples.

The MA5-13830 immunogen is full length recombinant human ERCC1 protein.

Target Information

ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. It is responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). It participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. (Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatin.

Experimental and therapeutic medicine

Liu L,Bai Z,Ma X,Wang T,Yang Y,Zhang Z

MA5-13830 was used in western blot to characterize chemosensitivity of SGC-7901 cells to oxaliplatin and the effects of taxol resistancne gene 1 expression

Tue Mar 01 00:00:00 EST 2016

The Concept of Divergent Targeting through the Activation and Inhibition of Receptors as a Novel Chemotherapeutic Strategy: Signaling Responses to Strong DNA-Reactive Combinatorial Mimicries.

Journal of signal transduction

Watt HL,Rachid Z,Jean-Claude BJ

MA5-13830 was used in immunocytochemistry and western blot to study the concept of divergent targeting through the activation and inhibition of receptors as a novel chemotherapeutic strategy

Thu Aug 23 00:00:00 EDT 2012

Delayed c-Fos activation in human cells triggers XPF induction and an adaptive response to UVC-induced DNA damage and cytotoxicity.

Cellular and molecular life sciences : CMLS

Tomicic MT,Reischmann P,Rasenberger B,Meise R,Kaina B,Christmann M

MA5-13830 was used in western blot to study the role of delayed c-Fos activation in the adaptive response to UVC-induced DNA damage and cytotoxicity

Sun May 01 00:00:00 EDT 2011

Subcellular distribution of a fluorescence-labeled combi-molecule designed to block epidermal growth factor receptor tyrosine kinase and damage DNA with a green fluorescent species.

Molecular cancer therapeutics

Todorova MI,Larroque AL,Dauphin-Pierre S,Fang YQ,Jean-Claude BJ

MA5-13830 was used in western blot to study the subcellular distribution of a mixed EGFR-DNA targeting molecule utilizing a green labelled DNA-damaging moiety and a blue-labelled EGFR inhibitor

Thu Apr 01 00:00:00 EDT 2010

The ERCC1/XPF endonuclease is required for completion of homologous recombination at DNA replication forks stalled by inter-strand cross-links.

Nucleic acids research

Al-Minawi AZ,Lee YF,Håkansson D,Johansson F,Lundin C,Saleh-Gohari N,Schultz N,Jenssen D,Bryant HE,Meuth M,Hinz JM,Helleday T

MA5-13830 was used in western blot to investigate the function of ERCC1-XPF complex in inter-strand cross-link repair during DNA replication

Thu Oct 01 00:00:00 EDT 2009

Development of an unsupervised pixel-based clustering algorithm for compartmentalization of immunohistochemical expression using Automated QUantitative Analysis.

Applied immunohistochemistry & molecular morphology : AIMM

Gustavson MD,Bourke-Martin B,Reilly DM,Cregger M,Williams C,Tedeschi G,Pinard R,Christiansen J

MA5-13830 was used in immunohistochemistry to evaluate a novel image processing algorithm for immunohistochemical analysis

Wed Jul 01 00:00:00 EDT 2009

ZNRD1 might mediate UV irradiation related DNA damage and repair in human esophageal cancer cells by regulation of ERCC1.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus

Guo W,Zhao YP,Jiang YG,Wang RW,Hong L,Fan DM

MA5-13830 was used in western blot to study the potential role of ZNRD1 in mediating UV irradiation-related DNA damage and repair in human esophageal cancer cells

Tue Mar 31 00:00:00 EDT 2009

ERCC1 expression and RAD51B activity correlate with cell cycle response to platinum drug treatment not DNA repair.

Cancer chemotherapy and pharmacology

Stordal B,Davey R

MA5-13830 was used in western blot to investigate the mechanism for platinum resistance in specific cell lines and the involvement of ERCC1 and RAD51B proteins

Sun Mar 01 00:00:00 EST 2009

Enhanced cisplatin cytotoxicity by disturbing the nucleotide excision repair pathway in ovarian cancer cell lines.

Cancer research

Selvakumaran M,Pisarcik DA,Bao R,Yeung AT,Hamilton TC

MA5-13830 was used in western blot to study the increased cytotoxicity of cisplatin following antisense knockdown of the nucleotide excision repair pathway in ovarian cancer cell lines

Sat Mar 15 00:00:00 EST 2003

Bioinformatics

Protein Aliases: DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)

Gene Aliases: COFS4; ERCC1; RAD10; UV20

UniProt ID: (Human) P07992

Entrez Gene ID: (Human) 2067

Molecular Function: endodeoxyribonuclease