|Flow Cytometry (Flow)||0.5-1ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Immunohistochemistry (IHC)||See 58 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 4 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunoprecipitation (IP)||See 3 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Hamster , Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant full length human ERCC1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13912 targets ERCC1 in IP, IHC (P), FACS and WB applications and shows reactivity with human, mouse and rat samples.
The MA5-13912 immunogen is recombinant full length human ERCC1 protein.
The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing 1; excision repair cross-complementing rodent repair deficiency, complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Gene Aliases: COFS4; Ercc-1; ERCC1; RAD10; UV20
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