|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues F(159) D S F D N D G K K N N P A I(173) of rat ERGIC-53.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA1-074 detects ERGIC-53 in rat, mouse and human samples.
PA1-074 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~57.5 kDa protein representing ERGIC-53 in rat brain samples.
The PA1-074 immunogen is a synthetic peptide corresponding to residues F(159) D S F D N D G K K N N P A I(173) of rat ERGIC-53. This sequence is conserved in mouse and human. The PA1-074 immunizing peptide (Cat. # PEP-277) is available for use in neutralization and control experiments.
Protein trafficking along the endoplasmic reticulum (ER) to the Golgi apparatus occurs by vesicular transport between membranous compartments. ERGIC-53 (ER-Golgi intermediate compartment 53) is a mannose-specific lectin transport receptor protein which cycles between the ER and the Golgi complex. ERGIC-53 is involved in intracellular transport of many glycoproteins. ERGIC-53 is useful in studying mechanisms underlying protein trafficking early in the secretory pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ADAM10 and BACE1 are localized to synaptic vesicles.
PA1-074 was used in western blot to localize ADAM10 and BACE1 in synaptic vesicles
|Lundgren JL,Ahmed S,Schedin-Weiss S,Gouras GK,Winblad B,Tjernberg LO,Frykman S||Journal of neurochemistry (135:606)||2015|
Synaptic and endosomal localization of active gamma-secretase in rat brain.
PA1-074 was used in western blot to study the subcellular localization of active gamma-secretase in rat brain
|Frykman S,Hur JY,Frånberg J,Aoki M,Winblad B,Nahalkova J,Behbahani H,Tjernberg LO||PloS one (5:null)||2010|