OriGene

ERI1 Monoclonal Antibody (OTI1B3), TrueMAB™

Product Image Gallery

ERI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-ERI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504134)

ERI1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERI1 (RC206777, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ERI1. Positive lysates LY407051 (100 µg) and LC407051 (20 µg) can be purchased separately from OriGene.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:500-1:2000

Product Specifications

Species

Human, Rat

Expression System

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI1B3

Immunogen

Full length human recombinant protein of human ERI1 produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3 ' exoribonuclease; 3' exoribonuclease; 3'-5' exonuclease ERI1; 3'-5' exoribonuclease 1; enhanced RNAi three prime mRNA exonuclease homolog 1; Eri-1 homolog; HEXO; histone mRNA 3' end-specific exonuclease; Histone mRNA 3'-end-specific exoribonuclease; Histone mRNA 3'-exonuclease 1; protei; Protein 3'hExo; three prime histone mRNA exonuclease 1; three prime mRNA exonuclease 1

Gene Aliases: 3'EXO; 3'HEXO; ERI1; HEXO; RGD1308378; THEX1

UniProt ID: (Human) Q8IV48, (Rat) Q5FVR4

Entrez Gene ID: (Human) 90459, (Rat) 361159

Molecular Function: RNA binding protein esterase exoribonuclease hydrolase