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Immunofluorescence analysis of ERK1 + ERK2 Antibody (ERK-7D8) was done on 70% confluent log phase U87-MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ERK1 + ERK2 Antibody (ERK-7D8)(136200) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic AND nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic Peptide (a.a. 324-345) corresponding to a segment near the C-terminus of rat ERK1.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Gel Shift (GS)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Extracellular signal-regulated kinase 2 (Erk2) is also known as mitogen activated protein kinase 2, microtubule-associated protein-2 kinase MAP kinase 2, p42-MAPK or EGF receptor T669 (ERT1). Mek2 activates Erk2 by phosphorylation of neighbouring threonine-183 and tyrosine-185 residues. Erk2, a serine-threonine kinase phosphorylates microtubule associated protein-2 and myelin basic protein. This kinase is an important proximal componant of the MAP kinase pathway involved in transmitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Oxidative pentose phosphate pathway inhibition is a key determinant of antimalarial induced cancer cell death.
13-6200 was used in western blot to show that inhibition of the oxidative arm of the pentose phosphate pathway is required for antimalarial-mediated apoptosis.
|Salas E,Roy S,Marsh T,Rubin B,Debnath J||Oncogene (35:2913)||2016|
ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function.
13-6200 was used in western blot to uncover a role for ATG12-ATG3 in late endosome function that is distinct from autophagy.
|Murrow L,Malhotra R,Debnath J||Nature cell biology (17:300)||2015|
Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells.
13-6200 was used in western blot to study the effect of Imatinib on VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells
|Bernusso VA,Machado-Neto JA,Pericole FV,Vieira KP,Duarte AS,Traina F,Hansen MD,Olalla Saad ST,Barcellos KS||Biochimica et biophysica acta (1853:388)||2015|
Primary murine CD4+ T cells fail to acquire the ability to produce effector cytokines when active Ras is present during Th1/Th2 differentiation.
13-6200 was used in western blot to investigate the role of Ras in the production of effector cytokines by primary murine CD4+ T cells during Th1/Th2 differentiation
|Janardhan SV,Marks R,Gajewski TF||PloS one (9:null)||2014|
Hinokitiol induces DNA damage and autophagy followed by cell cycle arrest and senescence in gefitinib-resistant lung adenocarcinoma cells.
13-6200 was used in western blot to study the effect of Hinokitiol on DNA damage and autophagy in gefitinib-resistant lung adenocarcinoma cells
|Li LH,Wu P,Lee JY,Li PR,Hsieh WY,Ho CC,Ho CL,Chen WJ,Wang CC,Yen MY,Yang SM,Chen HW||PloS one (9:null)||2014|
The effects of oral taurine administration on behavior and hippocampal signal transduction in rats.
13-6200 was used in western blot to study the effects of taurine on antidepressive behaviour and depression-related signaling in the rat hippocampus.
|Iio W,Matsukawa N,Tsukahara T,Toyoda A||Amino acids (43:2037)||2012|
|Human||Not Cited||Post-receptor IGF1 insensitivity restricted to the MAPK pathway in a Silver-Russell syndrome patient with hypomethylation at the imprinting control region on chromosome 11.||Montenegro LR,Leal AC,Coutinho DC,Valassi HP,Nishi MY,Arnhold IJ,Mendonca BB,Jorge AA||European journal of endocrinology / European Federation of Endocrine Societies (166:543)||2012|
Knockdown of insulin receptor substrate 1 reduces proliferation and downregulates Akt/mTOR and MAPK pathways in K562 cells.
13-6200 was used in western blot to determine the impact of IRS1 on the BCR-ABL signaling pathways.
|Machado-Neto JA,Favaro P,Lazarini M,Costa FF,Olalla Saad ST,Traina F||Biochimica et biophysica acta (1813:1404)||2011|
Insulin signaling in adipose tissue of patients with primary aldosteronism.
13-6200 was used in western blot to examine the phosphorylation of insulin-receptors substrate in various adenoma and adipocytes.
|Urbanet R,Pilon C,Giorgino F,Vettor R,Fallo F||Journal of endocrinological investigation (34:86)||2011|
|Mouse||Not Cited||ERK1/2 controls Na,K-ATPase activity and transepithelial sodium transport in the principal cell of the cortical collecting duct of the mouse kidney.||Michlig S,Mercier A,Doucet A,Schild L,Horisberger JD,Rossier BC,Firsov D||The Journal of biological chemistry (279:51002)||2004|
|Erk/Src phosphorylation of cortactin acts as a switch on-switch off mechanism that controls its ability to activate N-WASP.||Martinez-Quiles N,Ho HY,Kirschner MW,Ramesh N,Geha RS||Molecular and cellular biology (24:5269)||2004|
Blood lipid mediator sphingosine 1-phosphate potently stimulates platelet-derived growth factor-A and -B chain expression through S1P1-Gi-Ras-MAPK-dependent induction of Kruppel-like factor 5.
13-6200 was used in western blot to study SP()-triggered and G(i)-Ras-ERK/p38 MAPK-KLF5-dependent regulation of PDGF gene transcription in vascular smooth muscle cells.
|Usui S,Sugimoto N,Takuwa N,Sakagami S,Takata S,Kaneko S,Takuwa Y||The Journal of biological chemistry (279:12300)||2004|
|Rat||1:1000||Regulation of extracellular signal-regulated kinase by cannabinoids in hippocampus.||Derkinderen P,Valjent E,Toutant M,Corvol JC,Enslen H,Ledent C,Trzaskos J,Caboche J,Girault JA||The Journal of neuroscience : the official journal of the Society for Neuroscience (23:2371)||2003|
|Rat||Not Cited||Association of the Ras to mitogen-activated protein kinase signal transduction pathway with microfilaments. Evidence for a p185(neu)-containing cell surface signal transduction particle linking the mitogenic pathway to a membrane-microfilament association site.||Carraway CA,Carvajal ME,Carraway KL||The Journal of biological chemistry (274:25659)||1999|
|Rat||Not Cited||Hypothermic stress leads to activation of Ras-Erk signaling.||Chan EY,Stang SL,Bottorff DA,Stone JC||The Journal of clinical investigation (103:1337)||1999|
Stress-induced tau phosphorylation in mouse strains with different brain Erk 1 + 2 immunoreactivity.
13-6200 was used in western blot to compare the effect of cold water stress on the phosphorylation of brain tau protein in ob/ob and ob/+ mice.
|Korneyev AY||Neurochemical research (23:1539)||1998|
|Human||1:200||GIT1 is a scaffold for ERK1/2 activation in focal adhesions.||Yin G,Zheng Q,Yan C,Berk BC||The Journal of biological chemistry (280:27705)||2005|
||Association of the Ras to mitogen-activated protein kinase signal transduction pathway with microfilaments. Evidence for a p185(neu)-containing cell surface signal transduction particle linking the mitogenic pathway to a membrane-microfilament association site.||Carraway CA,Carvajal ME,Carraway KL||The Journal of biological chemistry (274:25659)||1999|
|Interleukin-1 represents a new modality for the activation of extracellular signal-regulated kinases/microtubule-associated protein-2 kinases.||Bird TA,Sleath PR,deRoos PC,Dower SK,Virca GD||The Journal of biological chemistry (266:22661)||1991|
ERK; ERK-2; ERK1; ERK2; ERT1; extracellular signal-regulated kinase 1; extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK; MAPK 1; MAPK 2; MAPK 3; MAPK2; microtubule-associated protein 2 kinase; Mitogen activated protein kinase; Mitogen activated protein kinase 1; Mitogen activated protein kinase 2; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; mitogen-activated protein kinase 3; p41; p41mapk; p42 MAP Kinase; p42-MAPK; P42MAPK; p44 MAP Kinase; pp42/MAP kinase; PRKM1; PRKM2; protein tyrosine kinase ERK2
9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3