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|Tested species reactivity||Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Primate, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||6-histidine epitope tagged full length recombinant rat ERp29.|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2,500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-011 detects ERp29 protein in rat, mouse, hamster, canine, non-human primate, bovine and guinea pig samples.
PA3-011 has successfully been used in immunofluorescence, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~29 kDa protein representing ERp29 in rat liver homogenate. Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using PA3-011.
The PA3-011 immunogen was a 6-histidine epitope tagged full length recombinant ERp29 protein.
Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be up regulated during ER stress conditions.
ERp29 is a 259-residue protein that is encoded by a gene on chromosome 12 in humans. This soluble protein is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI"e;s thioredoxin module.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Peripheral nerve axons contain machinery for co-translational secretion of axonally-generated proteins.
PA3-011 was used in immunocytochemistry to study the co-translational secretion of proteins synthesized in peripheral nerve axons
|Merianda T,Twiss J||Neuroscience bulletin (29:493)||2013|
PKR-like endoplasmic reticulum kinase (PERK) activation following brain ischemia is independent of unfolded nascent proteins.
PA3-011 was used in western blot to investigate the independence of PERK activation from unfolded nascent proteins
|Sanderson TH,Deogracias MP,Nangia KK,Wang J,Krause GS,Kumar R||Neuroscience (169:1307)||2010|
Differential cell death regulation between adult-unloaded and aged rat soleus muscle.
PA3-011 was used in western blot to study the different regulation of cell death in aged atrophy and un-load atrophy in rat skeletal muscle
|Ogata T,Machida S,Oishi Y,Higuchi M,Muraoka I||Mechanisms of ageing and development (130:328)||2009|
chromosome 12 open reading frame 8; endoplasmic reticulum lumenal protein ERp28; endoplasmic reticulum protein ERp29; endoplasmic reticulum resident protein 28; endoplasmic reticulum resident protein 29; endoplasmic reticulum resident protein 31; endoplasmic retuclum protein 29; epididymis secretory protein Li 107; ERp31; protein disulfide isomerase family A, member 9
1200015M03Rik; 2810446M09Rik; AW209030; BOS_16563; C12orf8; ERP28; ERP29; ERp31; HEL-S-107; PDI-DB; PDIA9