Invitrogen

ETS1 Monoclonal Antibody (8A8)

Product Image Gallery

ETS1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of NIH/3T3 cells using ETS1 monoclonal antibody (Product # MA5-15609) (Green). Blue: DRAQ5 fluorescent DNA dye.

ETS1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of paraffin-embedded human colon, adipocytes tissues using ETS1 monoclonal antibody (Product # MA5-15609).

ETS1 Antibody in Western Blot (WB) — Western blot analysis of ETS1 using ETS1 monoclonal antibody (Product # MA5-15609) in Jurkat (1), HepG2 (2) and ETS1-human IgG Fc transfected HEK293 (3) cell lysate.

ETS1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Jurkat cells using ETS1 monoclonal antibody (Product # MA5-15609) (green) and negative control (purple).

ETS1 Antibody in ChIP assay (ChIP) — Figure 3 STAT5 and TLX1 Co-bind Regulatory Regions throughout the Genome (A) Centered read density heatmaps of ChIPmentation ChIP-seq signals for the binding of TLX1, STAT5, p300, BRD4, ETS1, RUNX1, and the histone marks H3K27ac and H3K4me1. Heatmaps centered and ranked on TLX1 signal strength in leukemic NA + TLX1 and WT mouse cells. (B) GSEA comparing genes bound by both STAT5 and TLX1 and differentially expressed genes after treatment with imatinib in ALL-SIL cells. (C) Venn diagram showing the total amount of TLX1 and STAT5 peaks that fall within the 90,804 H3K27ac peaks for NA + TLX1 leukemic cells (left). Pie chart (right) showing ChIP-seq peak co-occurrence of STAT5 and TLX1 in mouse NA + TLX1 leukemic cells in relation to promoter regions (H3K4me3 + /H3K27ac + ) and enhancer regions (H3K27ac + /BRD4 + /p300 + /H3K4me3 - ). (D) Enhancer regions ranked on H3K27ac signal for NA + TLX1 leukemic cells and ALL-SIL. Only H3K27ac clusters with overlapping TLX1 peaks are shown. Gene labels are distance-based if not intragenic. (E-G) Representative ChIP-seq tracks for canonical STAT5 target genes Bcl2 (E), Pim1 (F), and Socs1 (G) showing STAT5, TLX1, H3K4me3, p300, BRD4, and H3K27ac binding in NA + TLX1 leukemic mouse cells.

Product Details

MA5-15609

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	1:200-1:1,000	-
Immunocytochemistry (ICC/IF)	1:200-1:1,000	-
Flow Cytometry (Flow)	1/200 - 1/400	View 1 publication 1 publication
ELISA (ELISA)	1:10,000	-
ChIP assay (ChIP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	8A8
Immunogen	Purified recombinant fragment of human ETS1 expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.03% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10978854

Product Specific Information

MA5-15609 targets ETS1 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15609 immunogen is purified recombinant fragment of human ETS1 expressed in E. Coli.
.

MA5-15609 detects ETS1 which has a predicted molecular weight of approximately 50.4kDa.

Target Information

ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homlog 1 (avian); ETS-1; EWSR2; p54), a 441 A. A protein, is a member of DNA-binding ETS protein family. It is a multifunctional protein consisting of an N-terminal pointed domain, a central transactivation domain and 2 auto inhibitory domains flanked on both sides of conserved C-terminal Ets domain. It interacts with factors including PEBP2alphaA, p300/CBP and mediates transactivation of Osteopontin, Presenilin-1, Stromelysin and Collagenase. It is activated by Ras/MAPK signaling and its expression is up-regulated in resting T-cells. Its activity is modulated by various factors including AP-1, Sp-1, c-myb and MafB. Sp100 activates ETS1 in nuclear bodies. It interacts with EAP1/Daxx and causes repression of MMP1 and BCL2 transactivation. It interacts with STAT6 and modulates Socs-1 expression induced by IL-4. It interacts with GATA3 and positively regulates Tax-1 dependent IL-5 expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cooperative Enhancer Activation by TLX1 and STAT5 Drives Development of NUP214-ABL1/TLX1-Positive T Cell Acute Lymphoblastic Leukemia.

Cancer cell

Vanden Bempt M,Demeyer S,Broux M,De Bie J,Bornschein S,Mentens N,Vandepoel R,Geerdens E,Radaelli E,Bornhauser BC,Kulozik AE,Meijerink JP,Bourquin JP,de Bock CE,Cools J

Published figure using ETS1 monoclonal antibody (Product # MA5-15609) in ChIP assay

Mon Aug 13 00:00:00 EDT 2018

Priming of transcriptional memory responses via the chromatin accessibility landscape in T cells.

Scientific reports

Tu WJ,Hardy K,Sutton CR,McCuaig R,Li J,Dunn J,Tan A,Brezar V,Morris M,Denyer G,Lee SK,Turner SJ,Seddiki N,Smith C,Khanna R,Rao S

MA515609 was used in flow cytometry to evaluate the mechanisms by which memory T cells exhibit transcriptional memory

Mon Mar 20 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; ets protein; Ets-1Delta(III-VI); FLJ10768; p54; Protein C-ets-1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1

Gene Aliases: c-ets-1; ETS-1; ETS1; EWSR2; p54

UniProt ID: (Human) P14921

Entrez Gene ID: (Human) 2113

Molecular Function: winged helix/forkhead transcription factor