Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (IHC) |
1:200-1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:200-1:1,000 | - |
Flow Cytometry (Flow) |
1/200 - 1/400 | View 1 publication 1 publication |
ELISA (ELISA) |
1:10,000 | - |
ChIP assay (ChIP) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
8A8 |
Immunogen |
Purified recombinant fragment of human ETS1 expressed in E. Coli. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
Conc. Not Determined |
Storage buffer |
ascites |
Contains |
0.03% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_10978854 |
MA5-15609 targets ETS1 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15609 immunogen is purified recombinant fragment of human ETS1 expressed in E. Coli.
.
MA5-15609 detects ETS1 which has a predicted molecular weight of approximately 50.4kDa.
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homlog 1 (avian); ETS-1; EWSR2; p54), a 441 A. A protein, is a member of DNA-binding ETS protein family. It is a multifunctional protein consisting of an N-terminal pointed domain, a central transactivation domain and 2 auto inhibitory domains flanked on both sides of conserved C-terminal Ets domain. It interacts with factors including PEBP2alphaA, p300/CBP and mediates transactivation of Osteopontin, Presenilin-1, Stromelysin and Collagenase. It is activated by Ras/MAPK signaling and its expression is up-regulated in resting T-cells. Its activity is modulated by various factors including AP-1, Sp-1, c-myb and MafB. Sp100 activates ETS1 in nuclear bodies. It interacts with EAP1/Daxx and causes repression of MMP1 and BCL2 transactivation. It interacts with STAT6 and modulates Socs-1 expression induced by IL-4. It interacts with GATA3 and positively regulates Tax-1 dependent IL-5 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; ets protein; Ets-1Delta(III-VI); FLJ10768; p54; Protein C-ets-1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1
Gene Aliases: c-ets-1; ETS-1; ETS1; EWSR2; p54
UniProt ID: (Human) P14921
Entrez Gene ID: (Human) 2113
Molecular Function:
winged helix/forkhead transcription factor
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