Invitrogen

EXO1 Monoclonal Antibody (266)

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EXO1 Antibody in Western Blot (WB) — Western blot analysis of Exo1 was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 10^5 mouse transformed pre-B cells. Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probe with a monoclonal anti-Exo1 antibody (Product # MA5-12262) at a dilution of 1:1000 in 2% milk/TBST at 4°C overnight, followed by a secondary antibody HRP-anti-rabbit at a dilution of 1:5000 at room temperature for 1 hour. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # PI-32209).

EXO1 Antibody in Immunocytochemistry (ICC/IF) — Figure 5. DNA-PK cs catalytic activity facilitates the initial recruitment of the DDR machinery to DSBs. ( A ) Recruitment of HR and NHEJ factors to the chromatin after IR is attenuated in the HCT116 DNA-PK cs KD/- cell line. HCT116 DNA-PK cs +/-, -/-, and KD/- cells were mock-treated or irradiated with a dose of 10 Gy and allowed to recover for 10 min. Subsequently, soluble nuclear and the chromatin-enriched fractions were isolated for immunoblotting to assess the recruitment of the proteins listed in the figure to the chromatin after irradiation. ( B ) IR-induced focus formation of MDC1 is attenuated in the HCT116 DNA-PK cs KD/- cell line. The HCT116 DNA-PK cs +/-, -/-, and KD/- cell lines were mock-treated or irradiated with a dose of 1 Gy and MDC1 foci formation was assessed 5 and 10 min later. MDC1 focus formation was examined in at least 50 cells and the number of MDC1 IR-induced foci per nucleus is shown. ****, P value < 0.0001. ( C - F ) Recruitment of GFP-tagged (C) NBS1, (D) EXO1, (E) XLF, and (F) XRCC4 to laser-generated DSBs is attenuated in the HCT116 DNA-PK cs KD/- cell line compared to the +/- cells. Relative fluorescent intensity of GFP-tagged NBS1, EXO1, XLF, and XRCC4 are presented as the mean +- SEM. *, P value < 0.05.

Product Details

MA5-12262

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	266
Immunogen	Recombinant full length mouse Exo1 protein View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	15mM sodium azide
Storage conditions	4° C
RRID	AB_10977111

Product Specific Information

MA5-12262 targets Exo1 in WB applications and shows reactivity with Human and mouse samples.

The MA5-12262 immunogen is recombinant full length mouse Exo1 protein.

Target Information

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Required for muscle atrophy following denervation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DNA-PKcs promotes chromatin decondensation to facilitate initiation of the DNA damage response.

Nucleic acids research

Lu H,Saha J,Beckmann PJ,Hendrickson EA,Davis AJ

Published figure using EXO1 monoclonal antibody (Product # MA5-12262) in Immunocytochemistry

Thu Oct 10 00:00:00 EDT 2019

Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1.

Nature structural & molecular biology

O'Sullivan RJ,Arnoult N,Lackner DH,Oganesian L,Haggblom C,Corpet A,Almouzni G,Karlseder J

MA5-12262 was used in western blot to study the effect of ASF1 depletion on the alternative lengthening of telomeres.

Sat Feb 01 00:00:00 EST 2014

Exonuclease 1 (Exo1) is required for activating response to S(N)1 DNA methylating agents.

DNA repair

Izumchenko E,Saydi J,Brown KD

MA5-12262 was used in immunoprecipitation and western blot to study the response to S(N)1 DNA methylating reagents and the role of exonuclease1 in activating the mismatch repair DNA damage response