Invitrogen

EXO1 Monoclonal Antibody (266)

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EXO1 Antibody in Western Blot (WB) — Western blot of Exo1 using Exo1 Monoclonal Antibody (Product # MA5-12262) on MAD109 Cells.

EXO1 Antibody in Western Blot (WB) — Western blot analysis of Exo1 was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 10^5 mouse transformed pre-B cells. Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probe with a monoclonal anti-Exo1 antibody (Product # MA5-12262) at a dilution of 1:1000 in 2% milk/TBST at 4°C overnight, followed by a secondary antibody HRP-anti-rabbit at a dilution of 1:5000 at room temperature for 1 hour. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # PI-32209).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

266

Immunogen

Recombinant full length mouse Exo1 protein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C

RRID

AB_10977111

Product Specific Information

MA5-12262 targets Exo1 in WB applications and shows reactivity with Human and mouse samples.

The MA5-12262 immunogen is recombinant full length mouse Exo1 protein.

Target Information

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Required for muscle atrophy following denervation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Exonuclease 1; Exonuclease I; hExo1; hExoI; mExo1; rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1

Gene Aliases: 5730442G03Rik; EXO1; EXOI; HEX1; hExoI; Msa

UniProt ID: (Human) Q9UQ84, (Mouse) Q9QZ11

Entrez Gene ID: (Human) 9156, (Mouse) 26909

Molecular Function: DNA binding protein damaged DNA-binding protein endodeoxyribonuclease exodeoxyribonuclease hydrolase nuclease nucleic acid binding

DNA synthesis involved in DNA repair strand displacement humoral immune response mediated by circulating immunoglobulin DNA replication DNA repair mismatch repair DNA recombination somatic hypermutation of immunoglobulin genes isotype switching meiotic cell cycle RNA phosphodiester bond hydrolysis, endonucleolytic regulation of signal transduction by p53 class mediator immune system process nucleobase-containing compound metabolic process cellular response to DNA damage stimulus nucleic acid phosphodiester bond hydrolysis

Process(es)

DNA binding chromatin binding RNA-DNA hybrid ribonuclease activity exonuclease activity protein binding 5'-3' exonuclease activity 5'-3' exodeoxyribonuclease activity single-stranded DNA 5'-3' exodeoxyribonuclease activity metal ion binding flap endonuclease activity double-stranded DNA 5'-3' exodeoxyribonuclease activity catalytic activity nuclease activity endonuclease activity hydrolase activity hydrolase activity, acting on ester bonds phosphoric ester hydrolase activity structure-specific DNA binding T/G mismatch-specific endonuclease activity retroviral 3' processing activity