Immunofluorescent analysis of EZH2/KMT6 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an EZH2/KMT6 polyclonal antibody (Product # PA5-29129) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 385 and 664 of Human EZH2/KMT6|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-Dependent|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29129 targets EZH2/KMT6 in WB applications and shows reactivity with Human samples.
The PA5-29129 immunogen is recombinant fragment corresponding to a region within amino acids 385 and 664 of Human EZH2/KMT6.
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.
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