|Tested species reactivity||Bovine, Dog, Horse, Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (473 YRKKGDSNSYNVRR 486) of Eck/EphA2/Mpk-5 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
EphA2 is a 976 amino acid oncoprotein belonging to the ephrin receptor subfamily of the protein-tyrosine kinase family. It consists of an external domain that includes a signal peptide, a transmembrane domain and a cytoplasmic domain that contains a canonical protein-tyrosine kinase catalytic domain. EphA2 are the largest receptor tyrosine kinase that binds ephrin-A ligands and play an important role in axon guidance and cell migration during development of the nervous system. Over expression of EphA2 is regulated by p53 and is known to be involved in tumor metastasis and angiogenesis. It is highly expressed in tissues that contain a high proportion of epithelial cells, including lung, skin, small intestine, and ovary and also in metastatic melanoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.