Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent staining of HeLa cells using PA5-19659, anti-Eg5 antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 5 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human Eg5.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EG5, HKSP, KNSL1, TRIP5, TR-interacting protein 5; kinesin-like 1; kinesin-like protein 1; kinesin-like protein KIF11; kinesin-like spindle protein HKSP; kinesin-related motor protein Eg5; thyroid receptor interacting protein 5; thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5
EG5; HKSP; KIF11; KNSL1; MCLMR; TRIP5