Western blot detection of Elg 1 in 5µg of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed using an Elg 1 polyclonal antibody (Product # PA1-31987) at a dilution of 1:500. The membrane was then washed and reacted with a HRP conjugated Goat-anti-Rabbit IgG (H and L) followed by ECL detection using a 2 min exposure time. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide (data not shown).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide LDYFRKTSPTNEKT-C conjugated to KLH, corresponding to amino acids 63-76 of Human Elg 1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31987 detects Elg 1 from human samples.
PA1-31987 has been successfully used in Western blot applications.
The PA1-31987 immunogen is: Synthetic peptide: LDYFRKTSPTNEKT-C conjugated to KLH, corresponding to amino acids 63-76 of Human Elg 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.