Immunofluorescent analysis of Endothelin 1 (green) showing positive staining in the secretion of HUVEC cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endothelin 1 monoclonal antibody (Product # MA3-005) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Sheep, Rat|
|Published species reactivity||Dog, Rat, Pig, Sheep, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Pure endothelin-1 conjugated to KLH.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/test|
|Immunohistochemistry (Frozen) (IHC (F))||1:250|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250|
|Radioimmune Assays (RIA)||1:25,000|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-005 detects ET-1 from human, sheep, mouse and rat tissues. This antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides.
MA3-005 has been successfully used in Western blot, flow cytometry, immunofluorescence, immunohistochemistry, immunocytochemistry, and radioimmune assay procedures. Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with MA3-005 results in staining of endothelial cells. Radioimmune assays using MA3-005 can be used to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine). This antibody is not recommended for human placenta tissue or HepG2 cells in Western blot applications.
For immunohistochemistry with MA3-005 formalin-fixed, paraffin-embedded tissue sections has been used.
The MA3-005 antigen is purified human endothelin-1. Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Endothelin-1 enriched tumor phenotype predicts breast cancer recurrence.
MA3-005 was used in immunohistochemistry to study the utility of endothelin-1 expression in tumor and stromal tissue for predicting breast cancer recurrence
|Tamkus D,Sikorskii A,Gallo KA,Wiese DA,Leece C,Madhukar BV,Chivu SC,Chitneni S,Dimitrov NV||ISRN oncology (2013:null)||2013|
Characterization of osteoblastic and osteolytic proteins in prostate cancer bone metastases.
MA3-005 was used in immunohistochemistry to characterize osteoblastic and osteolytic proteins in prostate cancer bone metastases
|Larson SR,Zhang X,Dumpit R,Coleman I,Lakely B,Roudier M,Higano CS,True LD,Lange PH,Montgomery B,Corey E,Nelson PS,Vessella RL,Morrissey C||The Prostate (73:932)||2013|
Effect of raloxifene and atorvastatin in atherosclerotic process in ovariectomized rats.
MA3-005 was used in immunohistochemistry to study the potential therapeutic effect of combined raloxifene and atorvastatin in atherosclerosis in aged ovariectomized rats
|Cetinkaya Demir B,Uyar Y,Ozbilgin K,Köse C||The journal of obstetrics and gynaecology research (39:229)||2013|
Decompression of the portal bed and twice-baseline portal inflow are necessary for the functional recovery of a "small-for-size" graft.
MA3-005 was used in immunohistochemistry to investigate the effect of a calibrated portocaval shunt on superfluous inflow in a porcine model of small-for-size syndrome
|Hessheimer AJ,Fondevila C,Taurá P,Muñoz J,Sánchez O,Fuster J,Rimola A,García-Valdecasas JC||Annals of surgery (253:1201)||2011|
CXCR6, a newly defined biomarker of tissue-specific stem cell asymmetric self-renewal, identifies more aggressive human melanoma cancer stem cells.
MA3-005 was used in immunohistochemistry to study CXCR6 as a marker of more aggressive human melanoma cancer stem cells
|Taghizadeh R,Noh M,Huh YH,Ciusani E,Sigalotti L,Maio M,Arosio B,Nicotra MR,Natali P,Sherley JL,La Porta CA||PloS one (5:null)||2010|
Clinicopathologic efficacy of copper bromide plus/yellow laser (578 nm with 511 nm) for treatment of melasma in Asian patients.
MA3-005 was used in immunohistochemistry to investigate the efficiency of laser treatment on melasma in Asians
|Lee HI,Lim YY,Kim BJ,Kim MN,Min HJ,Hwang JH,Song KY||Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.] (36:885)||2010|
Endothelin-1 expression correlates with atypical histological features in mammary phyllodes tumours.
MA3-005 was used in immunohistochemistry to study the correlation between endothelin-1 expression and atypical histological features in mammary phyllodes tumors
|Tse GM,Chaiwun B,Lau KM,Scolyer R,Lee CS,Karim RZ,Putti TC,Law BK,Lui PC,Tan PH||Journal of clinical pathology (60:1051)||2007|
Significance of enhanced expression of nitric oxide syntheses in splenic sinus lining cells in altered portal hemodynamics of idiopathic portal hypertension.
MA3-005 was used in immunohistochemistry to study the significance of enhanced expression of splenic sinus nitric oxide synthase in idiopathic portal hypertension
|Sato Y,Sawada S,Kozaka K,Harada K,Sasaki M,Matsui O,Nakanuma Y||Digestive diseases and sciences (52:1987)||2007|
The human placenta from heavy smokers: evaluation of vasoactive peptides by immunohistochemistry.
MA3-005 was used in immunohistochemistry to investigate the expression of e-NOS and endothelin-1 in human placenta from heavy smokers and non-smokers
|Clausen HV,Larsen LG,Jørgensen A,Bzorek M||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (115:22)||2007|
Comprehensive immunohistological analysis of the endothelin system in human kidney grafts.
MA3-005 was used in immunohistochemistry to investigate the expression of endothelin (ET)-1, ET-RA and ET-RB in the different cells and compartments of kidney grafts
|Frank K,Zeier M,Gross ML,Waldherr R,Ritz E,Amann K||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (21:1365)||2006|
Endothelin-1 promotes epithelial-to-mesenchymal transition in human ovarian cancer cells.
MA3-005 was used in immunohistochemistry to study the role of endothelin-1 in promoting epithelial-to-mesenchymal transition in human ovarian cancer cells
|Rosanò L,Spinella F,Di Castro V,Nicotra MR,Dedhar S,de Herreros AG,Natali PG,Bagnato A||Cancer research (65:11649)||2005|
Expression of the endothelin axis in bladder cancer: relationship to clinicopathologic parameters and long-term survival.
MA3-005 was used in immunohistochemistry to study the role of the endothelin axis in bladder cancer
|Wülfing C,Eltze E,Yamini J,Wülfing P,Bierer S,Böcker W,Hertle L,Semjonow A,Sievert KD||European urology (47:593)||2005|
Developmental changes in endothelial vasoactive and angiogenic growth factors in the human perinatal lung.
MA3-005 was used in immunohistochemistry to investigate the expression of endothelial vasoactive and angiogenic growth factors during human perinatal lung development
|Levy M,Maurey C,Chailley-Heu B,Martinovic J,Jaubert F,Israel-Biet D||Pediatric research (57:248)||2005|
Endothelin-1 and macrophage colony-stimulating factor are co-localized in human amnion membrane cells and secreted into amniotic fluid.
MA3-005 was used in immunohistochemistry to investigate the localization of endothelin-1 and macrophage colony-stimulating factor in human amnion membrane cells
|Fried G,Sand A,Ostlund E,Andersson E,Byström B,Ståbi B||Molecular human reproduction (9:719)||2003|
Knockout of insulin and IGF-1 receptors on vascular endothelial cells protects against retinal neovascularization.
MA3-005 was used in immunohistochemistry to study the role of insulin and IGF-1 signaling in retinal neovascularization.
|Kondo T,Vicent D,Suzuma K,Yanagisawa M,King GL,Holzenberger M,Kahn CR||The Journal of clinical investigation (111:1835)||2003|
Expression and regulation of endothelin-1 and its receptors in human penile smooth muscle cells.
MA3-005 was used in immunohistochemistry to study the expression of ET-1 and corresponding regulation and to identify its receptors in the human penis
|Granchi S,Vannelli GB,Vignozzi L,Crescioli C,Ferruzzi P,Mancina R,Vinci MC,Forti G,Filippi S,Luconi M,Ledda F,Maggi M||Molecular human reproduction (8:1053)||2002|
Use of in vitro organ cultures of human saphenous vein as a model for intimal proliferation.
MA3-005 was used in immunohistochemistry to generate organ cultures of human long saphenous vein for the study of intimal hyperplasia
|Porter KE||Methods in molecular biology (Clifton, N.J.) (206:199)||2002|
Pathogenic role of endothelin 1 in hemodynamic dysfunction in experimental acute pulmonary thromboembolism.
MA3-005 was used in immunohistochemistry to investigate the role of endothelin 1 in hemodynamic derangements of acute pulmonary thromboembolism
|Lee JH,Chun YG,Lee IC,Tuder RM,Hong SB,Shim TS,Lim CM,Koh Y,Kim WS,Kim DS,Kim WD,Lee SD||American journal of respiratory and critical care medicine (164:1282)||2001|
Endothelin receptor blockade inhibits proliferation of Kaposi's sarcoma cells.
MA3-005 was used in immunohistochemistry to investigate the impact of the endothelin receptor blockade on Kaposi's sarcoma cells.
|Bagnato A,Rosanò L,Di Castro V,Albini A,Salani D,Varmi M,Nicotra MR,Natali PG||The American journal of pathology (158:841)||2001|
Role of endothelin-1 in neovascularization of ovarian carcinoma.
MA3-005 was used in immunohistochemistry to study the role of endothelin 1 in ovarian cancer related-angiogenesis and its potential as a target of anti-angiogenic therapy.
|Salani D,Di Castro V,Nicotra MR,Rosanò L,Tecce R,Venuti A,Natali PG,Bagnato A||The American journal of pathology (157:1537)||2000|
Vascular remodeling and ET-1 expression in rat strains with different responses to chronic hypoxia.
MA3-005 was used in immunohistochemistry to investigate the vascular remodeling and expression of endothelin-1 in rat
|Aguirre JI,Morrell NW,Long L,Clift P,Upton PD,Polak JM,Wilkins MR||American journal of physiology. Lung cellular and molecular physiology (278:L981)||2000|
Expression of endothelin 1 and endothelin A receptor in ovarian carcinoma: evidence for an autocrine role in tumor growth.
MA3-005 was used in immunohistochemistry to study the endothelin 1 and the ETA receptor expression in primary and metastatic ovarian carcinomas and their involvement in tumor growth.
|Bagnato A,Salani D,Di Castro V,Wu-Wong JR,Tecce R,Nicotra MR,Venuti A,Natali PG||Cancer research (59:720)||1999|
Gene expression of endothelin-1, endothelin-converting enzyme-1, and endothelin receptors in human epididymis.
MA3-005 was used in immunohistochemistry to detect the expression of ET-1 in the human epididymis
|Peri A,Fantoni G,Granchi S,Vannelli GB,Barni T,Amerini S,Pupilli C,Barbagli G,Forti G,Serio M,Maggi M||The Journal of clinical endocrinology and metabolism (82:3797)||1997|
Endothelin-1 production and decreased endothelin B receptor expression in advanced prostate cancer.
MA3-005 was used in immunohistochemistry to detect the expression of endothelin-1 (ET-l) in advanced prostate cancer
|Nelson JB,Chan-Tack K,Hedican SP,Magnuson SR,Opgenorth TJ,Bova GS,Simons JW||Cancer research (56:663)||1996|
Rescue treatment with a Rho-kinase inhibitor normalizes right ventricular function and reverses remodeling in juvenile rats with chronic pulmonary hypertension.
MA3-005 was used in western blot to investigate the effect of ROCK inhibition on chronic pulmonary hypertension in rats
|Xu EZ,Kantores C,Ivanovska J,Engelberts D,Kavanagh BP,McNamara PJ,Jankov RP||American journal of physiology. Heart and circulatory physiology (299:H1854)||2010|
Green tea polyphenol epigallocatechin-3-gallate inhibits the endothelin axis and downstream signaling pathways in ovarian carcinoma.
MA3-005 was used in western blot to study the inhibitory effect of epigallocatechin-3-gallate on endothelin signaling pathways in ovarian carcinoma
|Spinella F,Rosanò L,Di Castro V,Decandia S,Albini A,Nicotra MR,Natali PG,Bagnato A||Molecular cancer therapeutics (5:1483)||2006|
Endothelial alterations during inhaled NO in lambs with pulmonary hypertension: implications for rebound hypertension.
MA3-005 was used in western blot to suggest that the endothelial response to iNO and the potential mechanisms of rebound pulmonary hypertension are dependent upon the underlying pulmonary vasculature
|Ross GA,Oishi P,Azakie A,Fratz S,Fitzgerald RK,Johengen MJ,Harmon C,Hendricks-Munoz K,Xu J,Black SM,Fineman JR||American journal of physiology. Lung cellular and molecular physiology (288:L27)||2005|
Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump.
MA3-005 was used in western blot to investigate the effect of ouabain on ET-1 expression and release in human endothelial cells
|Saunders R,Scheiner-Bobis G||European journal of biochemistry (271:1054)||2004|
Emergence of smooth muscle cell endothelin B-mediated vasoconstriction in lambs with experimental congenital heart disease and increased pulmonary blood flow.
MA3-005 was used in western blot to investigate the role of ETB receptors in the pathophysiology of pulmonary hypertension in lambs with experimental congenital heart disease and increased pulmonary blood flow
|Black SM,Mata-Greenwood E,Dettman RW,Ovadia B,Fitzgerald RK,Reinhartz O,Thelitz S,Steinhorn RH,Gerrets R,Hendricks-Munoz K,Ross GA,Bekker JM,Johengen MJ,Fineman JR||Circulation (108:1646)||2003|
Nitric oxide-endothelin-1 interactions after acute ductal constriction in fetal lambs.
MA3-005 was used in western blot to investigate the role of NO-ET-1 interactions in fetal pulmonary blood flow after acute mechanical constriction of the ductus arteriosus
|Ovadia B,Bekker JM,Fitzgerald RK,Kon A,Thelitz S,Johengen MJ,Hendricks-Munoz K,Gerrets R,Black SM,Fineman JR||American journal of physiology. Heart and circulatory physiology (282:H862)||2002|
Inhaled nitric oxide-induced rebound pulmonary hypertension: role for endothelin-1.
MA3-005 was used in western blot to investigate the role of endothelin-1 in the inhaled nitric oxide-induced rebound pulmonary hypertension
|McMullan DM,Bekker JM,Johengen MJ,Hendricks-Munoz K,Gerrets R,Black SM,Fineman JR||American journal of physiology. Heart and circulatory physiology (280:H777)||2001|
Endothelin: localization, synthesis, activity, and receptor types in human penile corpus cavernosum.
MA3-005 was used in immunocytochemistry to investigate the roles of endothelin in the regulation of human penile smooth muscle
|Saenz de Tejada I,Carson MP,de las Morenas A,Goldstein I,Traish AM||The American journal of physiology (261:H1078)||1991|