MA3-005 detects ET-1 from human, sheep, mouse and rat tissues. This antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides.
MA3-005 has been successfully used in Western blot, flow cytometry, immunofluorescence, immunohistochemistry, immunocytochemistry, and radioimmune assay procedures. Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with MA3-005 results in staining of endothelial cells. Radioimmune assays using MA3-005 can be used to concentrate ET-1 in solution (e.g. serµm/plasma, milk, urine). This antibody is not recommended for human placenta tissue or HepG2 cells in Western blot applications.
For immunohistochemistry with MA3-005 formalin-fixed, paraffin-embedded tissue sections has been used.
The MA3-005 antigen is purified human endothelin-1. Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Endothelin-1; ET-1; ppET-1; PPET1; preproendothelin; Preproendothelin-1
Gene Aliases: ARCND3; EDN1; ET-1; ET1; HDLCQ7; PPET-1; PPET1; preproET; QME